紫外分光光度-局部偏最小二乘回归法同时测定三种人造甜味剂

引用本文: 王海燕, 宋超, 张正勇, 谢伟量, 李丽萍, 刘军, 沙敏. 紫外分光光度-局部偏最小二乘回归法同时测定三种人造甜味剂[J]. 化学通报, 2016, 79(8): 744-748. shu

Citation: Wang Haiyan, Song Chao, Zhang Zhengyong, Xie Weiliang, Li Liping, Liu Jun, Sha Min. Simultaneous Determination of Three Artificial Sweeteners Using UV Spectroscopic Combined with Local Partial Least Squares Regression Method[J]. Chemistry, 2016, 79(8): 744-748. shu

紫外分光光度-局部偏最小二乘回归法同时测定三种人造甜味剂

王海燕 ^1,2, ,
宋超 ^1, ,
张正勇 ^1,2, ,
谢伟量 ^1,2, ,
李丽萍 ^3, ,
刘军 ^1,2, ,
沙敏 ^{1,2,
,}

1.
1. 南京财经大学管理科学与工程学院南京 210046;
2.
2. 江苏省质量安全工程研究院南京 210046;
3.
3. 南京理工大学机械工程学院南京 210094

通讯作者: 沙敏,E-mail:9120151037@njue.edu.cn

基金项目:
质检公益性行业科研专项（201410173)

国家自然科学基金项目（71433006、61373058）资助

摘要: 本文报道了一种简便、快速、准确地同时测定3种人造甜味剂安赛蜜、阿斯巴甜和糖精钠的方法。方法基于在pH为3.21的盐酸溶液中对安赛蜜、阿斯巴甜和糖精钠三组分混合溶液进行紫外光度测定，所得重叠光谱数据分别用偏最小二乘回归法（PLSR）、特征峰结合PLSR法和特征峰结合局部偏最小二乘回归法（LPLSR）进行处理。结果表明，选取特征波段的峰值作为自变量，采用4个局部样本做拟合的预报误差最小，总相对偏差仅为3.05%。对果汁样品进行测定，获得了很好的定量分析结果。安赛蜜、阿斯巴甜和糖精钠的定量线性范围分别为1.0～30.0 mg/L、1.0～10.0 mg/L和1.0～10.0 mg/L。

关键词:

English

Simultaneous Determination of Three Artificial Sweeteners Using UV Spectroscopic Combined with Local Partial Least Squares Regression Method

1.
1. School of Management Science & Engineering, Nanjing University of Finance & Economics, Nanjing 210046;
2.
2. Jiangsu Province Institute of Quality & Safety Engineering, Nanjing 210046;
3.
3. School of Mechanical Engineering, Nanjing University of Science & Technology, Nanjing 210094

Corresponding author: 沙敏,E-mail:9120151037@njue.edu.cn

Received Date: 19 January 2016
Fund Project:
质检公益性行业科研专项（201410173)

国家自然科学基金项目（71433006、61373058）资助

Abstract: Acesulfame-K, aspartame and saccharin sodium are commonly used as food flavoring agents. All of them show strong absorptions in the UV spectral region which are seriously overlapped. This paper reports simultaneous determination of them using UV spectroscopic combined with chemometric methods. Firstly, partial least squares regression (PLSR) was used, the total relative deviation was 7.01%. Secondly, PLSR combined with the characteristic peaks were employed, the total relative deviation was 4.67%. Lastly, local partial least squares regression (LPLSR) combined with the characteristic peaks were employed for the design of a model. The characteristic peaks of UV spectra of calibrating samples were chose as the independent variable factor, and LPLSR was used in data processing. Compared with PLSR method, the total relative deviation of LPLSR method is smaller, which is only 3.05%. Finally, the model was used to the concentration prediction of beverage samples with satisfactory results. The linear range of acesulfame-K, aspartame and saccharin sodium is 1.0~30.0 mg/L, 1.0~10.0 mg/L and 1.0~10.0 mg/L respctively.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

紫外分光光度-局部偏最小二乘回归法同时测定三种人造甜味剂

通讯作者: 沙敏,E-mail:9120151037@njue.edu.cn

English

Simultaneous Determination of Three Artificial Sweeteners Using UV Spectroscopic Combined with Local Partial Least Squares Regression Method

Corresponding author: 沙敏,E-mail:9120151037@njue.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

紫外分光光度-局部偏最小二乘回归法同时测定三种人造甜味剂

通讯作者: 沙敏,E-mail:9120151037@njue.edu.cn

English

Simultaneous Determination of Three Artificial Sweeteners Using UV Spectroscopic Combined with Local Partial Least Squares Regression Method

Corresponding author: 沙敏,E-mail:9120151037@njue.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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