引用本文:
邓慧敏, 黎军, 赖志辉, 赵善楷. 基体辅助激光解吸/电离质谱法对核酸分子的分析测定[J]. 应用化学,
1999, 16(1): 95-97.
Citation: Deng Huimin, Li Jun, Lai Zhihui, Zhao Shankai. Determination of Nucleotide Molecules by Matrixassisted Laser Desorption/Ionization Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, 1999, 16(1): 95-97.
Citation: Deng Huimin, Li Jun, Lai Zhihui, Zhao Shankai. Determination of Nucleotide Molecules by Matrixassisted Laser Desorption/Ionization Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, 1999, 16(1): 95-97.
基体辅助激光解吸/电离质谱法对核酸分子的分析测定
摘要:
基体辅助激光解吸/电离质谱(MALDI-MS)法自80年代末由Karas,Hillenkamp报道以来,已获得极大的发展.近几年来,其应用范围迅速扩大,各种生物大分子如蛋白质,核酸(DNA),多糖等都已能用M ALDI-MS法进行分子量测定.
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关键词:
- 基体辅助激光解吸电离质谱法
- / 核酸
- / 分子量测定
English
Determination of Nucleotide Molecules by Matrixassisted Laser Desorption/Ionization Mass Spectrometry
Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been used to determine the molecular weights of DNA oligomers d(T)6 and d(C)10.The results showed that 3-hydroxypicolinic acid was effective matrix in comparision with the mixture of 3-hydroxy picolinic acid and picolinic acid, 2, 5-dihydroxybenzoic acid, α-cyano-4-hydroxycinnamic acid, 2, 5-dihydroxyacetophenone and 2, 4, 6-trihydroxyacetophenone.NH4+ ion exchane resin and diammonium hydrogen citrate were used successfully to eliminate the influences of Na+, K+, Ca2+, Mg2+ ions.
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