引用本文:
祁超, 丁兰, 张玉静, 刘仔, 赵大庆. 人工模拟加卤酶Ⅱ.酶学性质[J]. 应用化学,
2001, 18(7): 560-562.
Citation: QI Chao, DING Lan, LIU Zhi, ZHANG Yu-Jing, ZHAO Da-Qing. Haloperoxidase by Imitation Ⅱ.The Enzymological Properties[J]. Chinese Journal of Applied Chemistry, 2001, 18(7): 560-562.
Citation: QI Chao, DING Lan, LIU Zhi, ZHANG Yu-Jing, ZHAO Da-Qing. Haloperoxidase by Imitation Ⅱ.The Enzymological Properties[J]. Chinese Journal of Applied Chemistry, 2001, 18(7): 560-562.
人工模拟加卤酶Ⅱ.酶学性质
摘要:
抗体酶具有比天然酶更高的立体结构专一性及稳定性,在医学、生物工程及工业上都有很大的应用潜力.
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关键词:
- 全抗原
- / 加卤酶
- / meso-四(T,T,T,T-O-苯乙酰苯)卟啉
- / 酶学性质
English
Haloperoxidase by Imitation Ⅱ.The Enzymological Properties
Abstract:
The meso-tetra(T,T,T,T-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques.The antibody could combine with Mn porphyrin and showed a haloperoxidase activity.The affinities of the antibody for antigen and Mn porphyrin were 4.98×10-5 mol/L and 7.63×10-5 mol/L, respectively.When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κcat for H2O2 were 5.127 0×10-3 mol/L and 0.4834 s-1, respectively.The enzyme exchange number κcat/Km=94.2851 mol/(L·s).
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