菠菜叶绿素的浸提和协同萃取反应

引用本文: 洪法水, 魏正贵, 赵贵文. 菠菜叶绿素的浸提和协同萃取反应[J]. 应用化学, 2001, 18(7): 532-535. shu

Citation: HONG Fa-Shui, WEI Zheng-Gui, ZHAO Gui-Wen. Soaking and Synergistic Extracting Reaction of Chlorophyll from Spinach[J]. Chinese Journal of Applied Chemistry, 2001, 18(7): 532-535. shu

菠菜叶绿素的浸提和协同萃取反应

1.
a 苏州大学生物科学系苏州 215151;
2.
b 中国科技大学化学系合肥
基金项目:
国家自然科学基金(29805003) (29805003)

苏州大学人才引进基金资助课题

摘要: 比较了不同有机溶剂用直接浸提法和Arnon法从菠菜叶片中提取叶绿素的效率.结果表明,用丙酮、乙醇浸提法提取叶绿素的效率比丙酮、乙醇Arno n法高,甲醇、乙醚浸提法提取叶绿素的效果明显不如丙酮、乙醇,而丙酮与乙醇等摩尔混合液浸提又比丙酮、乙醇液单独浸提的效率略高.对这种混合浸提现象的研究表明,在分配比为最大(D_max)时的乙醇浓度与混合液的总摩尔比为0.5、协萃系数R为1.85,认为用丙酮与乙醇混合液提取叶绿素存在协同效应,并且在两者等摩尔混合提取时效果最好.丙酮、乙醇及两者混合后与叶绿素的配位比均是1:1.

关键词:

叶绿素
/ 菠菜
/ 有机溶剂
/ 萃取

English

Soaking and Synergistic Extracting Reaction of Chlorophyll from Spinach

1.
a Department of Biological Science, Suzhou University, Suzhou 215151;
2.
b Department of Chemistry, China University of Science and Technology, Hefei
Received Date: 20 August 2000
Fund Project:
国家自然科学基金(29805003)

苏州大学人才引进基金资助课题

Abstract: The extraction efficiencies of chlorophyll from fresh spinach were compared on soaking method and Arnon methods using various organic solvents.The results showed that the extraction efficiencies of chlorophyll with soaking methods using acetone or ethanol were higher by 21%~38% than that with Arnon method.The extraction efficiencies of chlorophyll with soaking methods by acetone or ethanol were higher than that by methanol or ether.Soaking method using a mixture of acetone and ethanol showed higher(by 39%) efficiency than using acetone or ethanol alone.The soaking method using mixture solution of acetone and ethanol shows synergistic character.The studies of synergistic extraction indicated that the mole ratio of ethanol in mixture solution was 0.5 and synergistic coefficiency R was 1.85 at the maximum of distribution ratio, and coordination number was 1 for acetone, ethanol, and mixture of acetone and ethanol coordinated chlorophyll.

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沈阳化工大学材料科学与工程学院沈阳 110142

菠菜叶绿素的浸提和协同萃取反应

English

Soaking and Synergistic Extracting Reaction of Chlorophyll from Spinach

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通讯作者: 陈斌, bchen63@163.com

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菠菜叶绿素的浸提和协同萃取反应

English

Soaking and Synergistic Extracting Reaction of Chlorophyll from Spinach

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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