引用本文:
江虹, 胡小莉, 刘绍璞, 秦宗会, 湛海粼. 镧-依文思蓝-氨基糖苷类抗生素的显色反应及其分析应用[J]. 应用化学,
2003, 20(9): 883-887.
Citation: JIANG Hong, HU Xiao-Li, LIU Shao-Pu, QIN Zong-Hui, ZHAN Hai-Lin. Color Reaction of La and Evans Blue with Aminoglycoside Antibiotics and Its Analytical Applications[J]. Chinese Journal of Applied Chemistry, 2003, 20(9): 883-887.
Citation: JIANG Hong, HU Xiao-Li, LIU Shao-Pu, QIN Zong-Hui, ZHAN Hai-Lin. Color Reaction of La and Evans Blue with Aminoglycoside Antibiotics and Its Analytical Applications[J]. Chinese Journal of Applied Chemistry, 2003, 20(9): 883-887.
镧-依文思蓝-氨基糖苷类抗生素的显色反应及其分析应用
摘要:
在pH值为3.5~6.0,镧(Ⅲ)-依文思蓝(EB)与硫酸新霉素(NEO)、硫酸卡那霉素(KANA)、硫酸庆大霉(GEN)和硫酸妥布霉素(TOB)等氨基糖苷类抗生素反应可生成具有吸收峰和负吸收峰的三元蓝色离子缔合物。其最大吸收波长位于668~674nm,线性范围从0~1.20×10-5g/mL至0~1.40×10-5g/mL,摩尔吸收光系数(ε)在3.10×104~6.17×104L/(mol·cm)之间;最大负吸收波长位于614~620nm,线性范围从0~9.00×10-6g/mL至0~1.7×10-5g/mL,摩尔吸收光系数(ε)在4.33×104~1.04×105L/(mol·cm)。当用正负光吸收叠加时,灵敏度更高。探讨了反应条件、主要分析化学性质及三元缔合物的配合比(La:EB:GEN=1:2:3)。
English
Color Reaction of La and Evans Blue with Aminoglycoside Antibiotics and Its Analytical Applications
Abstract:
In the medium of pH 3.5~6.0, La(Ⅲ) and Evans blue(EB) react with aminoglycoside antibio-tics, such as neomycin sulfate(NEO), kanamycin sulfate(KANA), gentamycin sulfate(GEN) and tobramycin sulfate(TOB), to form ternary blue ion association complexes with an obvious absorption peak and an fading peak. The maximum absorption wavelengths are between 668 nm and 674 nm, the linear ranges in 0~1.2×10-5 g/mL to 0~1.40×10-5 g/mL, with molar absorptivities(ε) between 3.10×104 and 6.17×104 L/(mol·cm). The maximum fading wavelengths are in between 614 nm and 620 nm, and the linear ranges in 0~9.0×10-6 g/mL to 0~1.7×10-5 g/mL, with molar absorptivities(ε) between 4.33×104 and 1.04×105 L/(mol·cm). Their sensitivity is much higher when the positive minus wavelengths overlapping absorption spectrometry is used to determine the concentrations of aminoglycoside antibiotics. The reaction conditions, the important properties in analytical chemistry and the compound ratio of ternary ion association complexes(La:EB:GEN=1:2:3) have been discussed. The method has been applied to determining the content of the commerce aminoglycoside antibiotics with satisfactory results.
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Key words:
- La(Ⅲ)
- / evans blue
- / aminoglycoside
- / antibiotics
- / spectrophotometry
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