引用本文:
罗杨合, 蒋治良, 袁伟恩, 潘宏程, 唐国顺. HSA-硅钨杂多酸缔合纳米微粒体系的荧光猝灭[J]. 应用化学,
2003, 20(9): 833-836.
Citation: LUO Yang-He, JIANG Zhi-Liang, YUAN Wei-En, PAN Hong-Cheng, TANG Guo-Shun. Fluorescence Quenching of HSA-Silicotungstic Heteropolyacid Associated Nanoparticle System[J]. Chinese Journal of Applied Chemistry, 2003, 20(9): 833-836.
Citation: LUO Yang-He, JIANG Zhi-Liang, YUAN Wei-En, PAN Hong-Cheng, TANG Guo-Shun. Fluorescence Quenching of HSA-Silicotungstic Heteropolyacid Associated Nanoparticle System[J]. Chinese Journal of Applied Chemistry, 2003, 20(9): 833-836.
HSA-硅钨杂多酸缔合纳米微粒体系的荧光猝灭
English
Fluorescence Quenching of HSA-Silicotungstic Heteropolyacid Associated Nanoparticle System
Abstract:
In a Tris buffer solution(pH=7.40), there are an absorption peak at 260 nm for silicotungstic heteropolyacid(SiW) system and independently a fluorescence peak at 350 nm for human serum albumin(HSA) system. When both HSA and SiW solutions are mixed, ion association nanoparticles in size of 50 nm are formed. As a result Rayleigh scattering peak at 470 nm was enhanced considerably and the fluorescence peak at 350 nm was quenched. The Rayleigh scattering spetra and TEM observation revealed that the formation of HSA-SiW nanoparticles and interface formation are main factors that causes the fluorescence quenching and Rayleigh scattering enhancement.
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