Abstract: The solid-liquid surface reaction method was employed for the preparation of diallyl trisulfide-bonded silica gel (DTS).The γ-mercaptopropyl-bonded silica gel (MPS) was firstly obtained with coupling reagent and silica gel.Then diallyl trisulfide-bonded silica gel (DTS) was prepared by the mercapto-alkylene addition reaction between coupling reagent silica gel and diallyl trisulfide in the presence of initiator.The surface structure of DTS was characterized by elemental analysis,FT-IR,thermal analysis and electrospray mass spectrometry.The bonded amounts of MPS and DTS were 1.73 mmol/g and 0.27 mmol/g,respectively.The antibacterial property of prepared material DTS was investigated via antibacterium-circle.The antibacterium-circles of DTS to Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Aerobacter aerogenes and Proteus vulgaris were 9.0 mm,8.1 mm,10.1 mm,9.2 mm and 8.9 mm,respectively.The influence of the content of DTS in beef extract peptone medium on the inhibition effect to Staphylococcus aureus and Escherichia coli by mixing-smearing plate cultivate method was also investigated.The results showed that DTS exhibits a good inhibition effect on these five bacteria.After chemically bonding,not only elimination of the strong odour of Allium Sativum L,but also preservation of the antibacterial activity were found on the surface of DTS.Meanwhile,the antibacterial mechanism of DTS was proposed based on the experimental result analysis.A mixing-smearing plate culture method can be provided for the antibacterial test of the hydrophobic particle samples.