引用本文:
王欣, 贾丽华, 郭祥峰, 卢小霞. α-硫辛酸与牛血清白蛋白的相互作用[J]. 应用化学,
2007, 24(7): 761-764.
Citation: WANG Xin, JIA Li-Hua, GUO Xiang-Feng, LU Xiao-Xia. Interaction Between Alpha-Lipoic Acid and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2007, 24(7): 761-764.
Citation: WANG Xin, JIA Li-Hua, GUO Xiang-Feng, LU Xiao-Xia. Interaction Between Alpha-Lipoic Acid and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, 2007, 24(7): 761-764.
α-硫辛酸与牛血清白蛋白的相互作用
摘要:
利用荧光光谱和紫外-可见吸收光谱研究了在缓冲溶液中不同温度下α-硫辛酸(ALA)与牛血清白蛋白(BSA)的相互作用。结果表明,ALA对BSA的内源荧光猝灭为静态猝灭过程,猝灭常数KSV分别为4.65×103 L/mol (26℃)和4.46×103 L/mol (37℃)。依据Förster非辐射能量转移机制,得到给体(BSA)-受体(ALA)间的结合距离r=2.90 nm,能量转移效率E=5%。测定了该反应在不同温度下的结合常数KA=4.31×103 L/mol (26℃),4.27×103 L/mol (37℃),以摩尔比1:1结合。根据不同温度下的结合常数确定了相互作用过程的热力学参数,并根据热力学参数确定了ALA与BSA之间作用力主要是静电作用。
English
Interaction Between Alpha-Lipoic Acid and Bovine Serum Albumin
Abstract:
The interaction between alpha-lipoic acid (ALA) and bovine serum albumin (BSA) was studied via fluorescence and ultraviolet-visible absorption spectra in buffer at different temperatures.The results indicate that static quenching was involved in added ALA in BSA solution and the quenching constant (KSV) was 4.65×103 L/mol (26℃) and 4.46×103 L/mol (37℃) respectively.The energy transfer efficiency E is 5% and the distance r is 2.90 nm between drug and serum albumin according to Förster's non-radiation energy transfer.The binding constants is 4.31×103 L/mol (26℃) and 4.27×103 L/mol (37℃) respectively and they react at a molar ratio of 1:1.The interaction between alpha-lipoic acid and bovine serum albumin is driven mainly by eletro-static force according to thermodynamic parameters.
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Key words:
- alpha lipoic acid
- / bovine serum albumin
- / fluorescence quenching
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