A two-photon mitotracker based on a naphthalimide fl uorophore：Synthesis, photophysical properties and cell imaging

注册 English

A two-photon mitotracker based on a naphthalimide fl uorophore：Synthesis, photophysical properties and cell imaging

Yong Dai , Bao-Kuo Lv , Xin-Fu Zhang , Yi Xiao

Citation: Yong Dai, Bao-Kuo Lv, Xin-Fu Zhang, Yi Xiao. A two-photon mitotracker based on a naphthalimide fl uorophore：Synthesis, photophysical properties and cell imaging[J]. Chinese Chemical Letters, 2014, 25(7): 1001-1005. doi: 10.1016/j.cclet.2014.05.020 shu

shu

A two-photon mitotracker based on a naphthalimide fl uorophore：Synthesis, photophysical properties and cell imaging

Yong Dai ^a,c, ,
Bao-Kuo Lv ^{b,
,} ,
Xin-Fu Zhang ^c, ,
Yi Xiao ^{c,
,}

通讯作者: Bao-Kuo Lv,
Yi Xiao,

基金项目:
We thank National Natural Science Foundation of China (Nos. 21174022, 21376038) (Nos. 21174022, 21376038)

National Basic Research Program of China (No. 2013CB733702) (No. 2013CB733702)

Key Project of the Education Department of Sichuan Province (No. 12ZA087) (No. 12ZA087)

Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110041110009). (No. 20110041110009)

摘要: PAHPN, a naphthalimide-based mitotracker with reasonable two-photon excitation emission activity and polarity-sensitive fluorescence properties has been efficiently synthesized and studied in twophoton, co-localization, and FLIM imaging.

关键词:

English

A two-photon mitotracker based on a naphthalimide fl uorophore：Synthesis, photophysical properties and cell imaging

Yong Dai ^a,c, ,
Bao-Kuo Lv ^{b,
,} ,
Xin-Fu Zhang ^c, ,
Yi Xiao ^{c,
,}

1.
a Department of Criminal Science and Technology, Sichuan Police College, Luzhou 646000, China;
2.
b Liaoning Water Environment Monitoring Center, Shenyang Branch, Shenyang 110005, China;
3.
c State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

Corresponding author: Bao-Kuo Lv, Yi Xiao,

Received Date: 27 March 2014
Fund Project:

Abstract: PAHPN, a naphthalimide-based mitotracker with reasonable two-photon excitation emission activity and polarity-sensitive fluorescence properties has been efficiently synthesized and studied in twophoton, co-localization, and FLIM imaging.

Key words:

1. [1] K. Henze, W. Martin, Evolutionary biology: essence of mitochondria, Nature 426 (2003) 127-128.[1] K. Henze, W. Martin, Evolutionary biology: essence of mitochondria, Nature 426 (2003) 127-128.
2. [2] H.M. McBride, M. Neuspiel, S. Wasiak, Mitochondria: more than just a powerhouse, Curr. Biol. 16 (2006) 551-560.[2] H.M. McBride, M. Neuspiel, S. Wasiak, Mitochondria: more than just a powerhouse, Curr. Biol. 16 (2006) 551-560.
3. [3] D.R. Green, J.C. Reed, Mitochondria and apoptosis, Science 281 (1998) 1309-1312.[3] D.R. Green, J.C. Reed, Mitochondria and apoptosis, Science 281 (1998) 1309-1312.
4. [4] J. Bereiter-Hahn, M. Vösth, Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria, Microsc. Res. Tech. 27 (1994) 198-219.[4] J. Bereiter-Hahn, M. Vösth, Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria, Microsc. Res. Tech. 27 (1994) 198-219.
5. [5] R.J. Youle, M. Karbowski, Mitochondrial fission in apoptosis, Nat. Rev. Mol. Cell Biol. 6 (2005) 657-663.[5] R.J. Youle, M. Karbowski, Mitochondrial fission in apoptosis, Nat. Rev. Mol. Cell Biol. 6 (2005) 657-663.
6. [6] B. Chazotte, Labeling mitochondria with mitotracker dyes, Cold Spring Harb. Protoc. 8 (2011) 990-992.[6] B. Chazotte, Labeling mitochondria with mitotracker dyes, Cold Spring Harb. Protoc. 8 (2011) 990-992.
7. [7] F. Helmchen, W. Denk, Deep tissue two-photon microscopy, Nat. Methods 15 (2005) 932-934.[7] F. Helmchen, W. Denk, Deep tissue two-photon microscopy, Nat. Methods 15 (2005) 932-934.
8. [8] C.S. Lim, B.R. Cho, Two-photon probes for biomedical applications, BMB Rep. 46 (2013) 188-194.[8] C.S. Lim, B.R. Cho, Two-photon probes for biomedical applications, BMB Rep. 46 (2013) 188-194.
9. [9] H. Yu, Y. Xiao, L. Jin, A lysosome-targetable and two-photon fluorescent probe for monitoring endogenous and exogenous nitric oxide in living cells, J. Am. Chem. Soc. 134 (2012) 17486-17489.[9] H. Yu, Y. Xiao, L. Jin, A lysosome-targetable and two-photon fluorescent probe for monitoring endogenous and exogenous nitric oxide in living cells, J. Am. Chem. Soc. 134 (2012) 17486-17489.
10. [10] M.F. Ross, G.F. Kelso, F.H. Blaikie, et al., Lipophilic triphenylphosphonium cations as tools in mitochondrial bioenergetics and free radical biology, Biochemistry (Mosc.) 70 (2005) 222-230.[10] M.F. Ross, G.F. Kelso, F.H. Blaikie, et al., Lipophilic triphenylphosphonium cations as tools in mitochondrial bioenergetics and free radical biology, Biochemistry (Mosc.) 70 (2005) 222-230.
11. [11] B.C. Dickinson, C.J. Chang, A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells, J. Am. Chem. Soc. 130 (2008) 9638-9639.[11] B.C. Dickinson, C.J. Chang, A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells, J. Am. Chem. Soc. 130 (2008) 9638-9639.
12. [12] X.F. Guo, X.H. Qian, L.H. Jia, A highly selective and sensitive fluorescent chemosensor for Hg²⁺ in neutral buffer aqueous solution, J. Am. Chem. Soc. 126 (2004) 2272-2273.[12] X.F. Guo, X.H. Qian, L.H. Jia, A highly selective and sensitive fluorescent chemosensor for Hg²⁺ in neutral buffer aqueous solution, J. Am. Chem. Soc. 126 (2004) 2272-2273.
13. [13] W. Rettig, R. Lapouyade, Fluorescence probes based on twisted intramolecular charge transfer (TICT) states and other adiabatic photoreactions, Top. Fluoresc. Spectrosc. 4 (1994) 109-149.[13] W. Rettig, R. Lapouyade, Fluorescence probes based on twisted intramolecular charge transfer (TICT) states and other adiabatic photoreactions, Top. Fluoresc. Spectrosc. 4 (1994) 109-149.

WeChat

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 1501
HTML全文浏览量: 31

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

/

下载: 全尺寸图片幻灯片

分享

用微信扫码二维码

分享至好友和朋友圈