微流蒸发光散射检测器的研制及评价

引用本文: 王玉红, 刘芳, 赵卉, 王建民, 王彦, 阎超. 微流蒸发光散射检测器的研制及评价[J]. 分析化学, 2012, 40(10): 1622-1626. doi: 10.3724/SP.J.1096.2012.20373 shu

Citation: WANG Yu-Hong, LIU Fang, ZHAO Hui, WANG Jian-Min, WANG Yan, YAN Chao. Development and Evaluation of Micro Evaporative Light Scattering Detector[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1622-1626. doi: 10.3724/SP.J.1096.2012.20373 shu

微流蒸发光散射检测器的研制及评价

王玉红 ^1,2,3, ,
刘芳 ^2, ,
赵卉 ^2, ,
王建民 ^3, ,
王彦 ^2, ,
阎超 ^{1,2,3,
,}

通讯作者: 阎超

基金项目:
本文系国家重大科学仪器设备开发专项项目(No.2011YQ150072) (No.2011YQ150072)

国家自然科学基金项目(No.21175092) (No.21175092)

上海市引进技术的吸收与创新计划项目(No.JJ-YJCX-03-10-2586)资助 (No.JJ-YJCX-03-10-2586)

摘要: 构建了适用于纳升级到微升级流量的毛细管分离体系的微流蒸发光散射检测器(μELSD),实现了其与毛细管液相色谱(cLC)的联用。对雾化器孔径和雾化毛细管内径、蒸发管内径和长度、光散射池尺寸、雾化毛细管位置和辅助载气流量等参数进行了优化。在最优条件下,微流蒸发光散射检测器检出限为直接进样葡萄糖1 ng(S/N＞10),线性范围0.01-1.0 μg,重复性好,峰面积RSD(n=6) 为0.4%,峰高RSD(n=6)为0.3%。本检测器已成功应用cLC-μELSD平台,使用C₁₈毛细管色谱柱(内径250 μm),0.1%甲酸铵溶液(pH 4.5)-甲醇(60:40,V/V)为流动相,分离检测了3种常用甜味剂,表明本研究构建的系统可以应用于实际分离检测中,具有分析时间快、溶剂消耗量少、样品需求量小的优点。

关键词:

English

Development and Evaluation of Micro Evaporative Light Scattering Detector

1.
¹ Department of Chemistry, East China Normal University, Shanghai 200062, China;

Corresponding author: 阎超

Received Date: 09 April 2012
Fund Project:
本文系国家重大科学仪器设备开发专项项目(No.2011YQ150072)

国家自然科学基金项目(No.21175092)

上海市引进技术的吸收与创新计划项目(No.JJ-YJCX-03-10-2586)资助

Abstract: A novel micro-flow evaporative light scattering detector (μELSD), suitable for capillary liquid chromatography (cLC) system, was constructed and eveluated. The main parameters of detector, such as the aperture of nebulizer, inner diameter of capillary, specification of the evaporative tube and scattering cell, were optimized. Under the optimal experimental conditions:nebulizer 410 μm I.D., nebulized capillary 50 μm I.D.×360 μm O.D., evaporative tube 15 cm×4.6 mm I.D., scattering cell 60 mm×33 mm I.D., the detection limitation of glucose (S/N＞10) was 1 ng with a linear range of 0.01-1 μg. Good reproducibility has been achieved in peak area and peak height with the RSD (n=6) of 0.4% and 0.3% (n=6), respectively. The detector was successfully coupled to a cLC system and applied to the separation and detection of three kinds of sweeteners, in the condition of capillary column ( C₁₈, 250 μm I.D.) and 0.1% (V/V) ammonium formate (pH=4.5): methanol (60:40, V/V) as mobile phase. The cLC-μELSD system has the advantages in universal applicability, high column efficiency, and less solvent and sample consumptions compare with a common HPLC-ELSD system, and is suitable for separation and analysis of real samples.

Key words:

1. [1]
  1 Chariesworth J M. Anal. Chem., 1978, 50: 1414-14201 Chariesworth J M. Anal. Chem., 1978, 50: 1414-1420
2. [2]
  2 Stolyhwo A, Colin H, Guiochon G. J. Chromatogr., 1983, 265: 1-182 Stolyhwo A, Colin H, Guiochon G. J. Chromatogr., 1983, 265: 1-18
3. [3]
  3 Stolyhwo A, Colin H, Martin M, Guiochon G. J. Chromatogr., 1984, 288: 253-2753 Stolyhwo A, Colin H, Martin M, Guiochon G. J. Chromatogr., 1984, 288: 253-275
4. [4]
  4 Stolyhwo A, Colin H, Guiochon G. Anal. Chem., 1985, 57: 1342-13544 Stolyhwo A, Colin H, Guiochon G. Anal. Chem., 1985, 57: 1342-1354
5. [5]
  5 ZHAO Shao-Hua, LIU Min-Yan, WANG Yu-Feng, XU Hong-Hhui, ZHANG Yong-Feng, WANG Meng. Food Science, 2012, 33(02): 224-2265 ZHAO Shao-Hua, LIU Min-Yan, WANG Yu-Feng, XU Hong-Hhui, ZHANG Yong-Feng, WANG Meng. Food Science, 2012, 33(02): 224-226
6. [6]
  赵韶华, 刘敏彦, 王玉峰, 许红辉, 张永锋, 王猛. 食品科学, 2012, 33(2): 224-226赵韶华, 刘敏彦, 王玉峰, 许红辉, 张永锋, 王猛. 食品科学, 2012, 33(2): 224-226
7. [7]
  6 LIU Fang, WANG Yan, WANG Yu-Hong, ZHOU Jun-Yi, YAN Chao. Chinese Journal of Chromatography, 2012, 30(3): 292-2976 LIU Fang, WANG Yan, WANG Yu-Hong, ZHOU Jun-Yi, YAN Chao. Chinese Journal of Chromatography, 2012, 30(3): 292-297
8. [8]
  刘芳, 王彦, 王玉红, 周君裔, 阎超. 色谱, 2012, 30(3): 292-297刘芳, 王彦, 王玉红, 周君裔, 阎超. 色谱, 2012, 30(3): 292-297
9. [9]
  7 LIU Wei, JIN Cheng, KONG Wei-Jun, WANG Hai-Tao, GONG Qian-Feng, XIAO Xiao-He. Chinese J. Anal. Chem., 2010, 38(4):522-5267 LIU Wei, JIN Cheng, KONG Wei-Jun, WANG Hai-Tao, GONG Qian-Feng, XIAO Xiao-He. Chinese J. Anal. Chem., 2010, 38(4):522-526
10. [10]
  刘苇, 金城, 孔维军, 王海涛, 龚千锋, 肖小河. 分析化学, 2010, 38(4): 522-526刘苇, 金城, 孔维军, 王海涛, 龚千锋, 肖小河. 分析化学, 2010, 38(4): 522-526
11. [11]
  8 WANG Yu-Hong, SHEN Ke-Yu, LI Peng, ZHOU Jun-Yi, YAN Chao. Chinese Journal of Chromatography, 2011, 29(9): 908-9118 WANG Yu-Hong, SHEN Ke-Yu, LI Peng, ZHOU Jun-Yi, YAN Chao. Chinese Journal of Chromatography, 2011, 29(9): 908-911
12. [12]
  王玉红, 申克宇, 李鹏, 周君裔, 阎超. 色谱, 2011, 29(9): 908-911王玉红, 申克宇, 李鹏, 周君裔, 阎超. 色谱, 2011, 29(9): 908-911
13. [13]
  9 ZHANG Yong, ZHOU Ya-Qiu, CHEN Fa-Gui.Chinese Journal of Pharmaceutical Analysis, 2008, 28(10): 1732-17349 ZHANG Yong, ZHOU Ya-Qiu, CHEN Fa-Gui.Chinese Journal of Pharmaceutical Analysis, 2008, 28(10): 1732-1734
14. [14]
  张勇, 周亚球, 陈法贵. 药物分析杂志, 2008, 28(10): 1732-1734张勇, 周亚球, 陈法贵. 药物分析杂志, 2008, 28(10): 1732-1734
15. [15]
  10 James N A. J. Microcolumn Separations, 1998, 10(6): 491-50210 James N A. J. Microcolumn Separations, 1998, 10(6): 491-502
16. [16]
  11 Magnus B O A, Lars G B. J. Microcolumn Separations, 1998, 10(3): 249-25411 Magnus B O A, Lars G B. J. Microcolumn Separations, 1998, 10(3): 249-254
17. [17]
  12 Karen G, Arlette B, Pierre C. J. Chromatogr. A, 2004, 1051(1-2): 43-5112 Karen G, Arlette B, Pierre C. J. Chromatogr. A, 2004, 1051(1-2): 43-51
18. [18]
  13 Loic Q, Karen G, Arlette B, Pierre C. J. Separation Science, 2006, 29(3): 390-39813 Loic Q, Karen G, Arlette B, Pierre C. J. Separation Science, 2006, 29(3): 390-398
19. [19]
  14 Guillarme D, Rudaz S, Schelling C, Dreux M, Veuthey J L. J. Chromatogr. A, 2008, 1192(1): 103-11214 Guillarme D, Rudaz S, Schelling C, Dreux M, Veuthey J L. J. Chromatogr. A, 2008, 1192(1): 103-112

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

微流蒸发光散射检测器的研制及评价

通讯作者: 阎超

English

Development and Evaluation of Micro Evaporative Light Scattering Detector

Corresponding author: 阎超

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

微流蒸发光散射检测器的研制及评价

通讯作者: 阎超

English

Development and Evaluation of Micro Evaporative Light Scattering Detector

Corresponding author: 阎超

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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