顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物

引用本文: 肖天鹏, 郑晓峰, 吕吉鸿, 郭泽峰, 高峰, 刘春凤, 李崎. 顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物[J]. 分析化学, 2012, 40(10): 1589-1592. doi: 10.3724/SP.J.1096.2012.20324 shu

Citation: XIAO Tian-Peng, ZHENG Xiao-Feng, LV Ji-Hong, GUO Ze-Feng, GAO Feng, LIU Chun-Feng, LI Qi. Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1589-1592. doi: 10.3724/SP.J.1096.2012.20324 shu

顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物

肖天鹏 ^1,2, ,
郑晓峰 ^3, ,
吕吉鸿 ^3, ,
郭泽峰 ^3, ,
高峰 ^3, ,
刘春凤 ^1,2, ,
李崎 ^{1,2,
,}

通讯作者: 李崎

基金项目:
本文系国家"十一五"支撑重点项目(No.2008BAI63B06)资助 (No.2008BAI63B06)

摘要: 建立了顶空-固相微萃取(HS-SPME)-气相色谱快速测定可可麦汁中3种吡嗪类物质(2,5-二甲基吡嗪、2,3,5-三甲基吡嗪和2,3,5,6-四甲基吡嗪)的方法。选择不同的固相微萃取头对萃取温度和时间进行优化,所得最佳萃取条件为:在60℃下,采用75 μm CAR/PDMS萃取头对麦汁样品萃取40 min。本方法的检出限(S/N＝3)为0.023-0.056 μg/L,线性范围1-500 mg/L;相对标准偏差为3.6%-6.4%;回收率为95.4%-102.7%。本方法应用于样品检测,发现可可麦汁中吡嗪的浓度与原料中可可粉的添加量正相关,显示了很好灵敏性。

关键词:

English

Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction

1.
¹ The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

Corresponding author: 李崎

Received Date: 28 March 2012
Fund Project:
本文系国家"十一五"支撑重点项目(No.2008BAI63B06)资助

Abstract: A headspace solid phase microextraction (HS-SPME), followed by gas chromatographic method was established for the detection of three pyrazine compounds (2,5-dimethyl pyrazine, 2,3,5-trimethyl pyrazine and 2,3,5,6-tetramethyl pyrazine) in cocoa wort. Equilibrium temperature and equilibrium time were optimized by using different SPME heads. According to the results of the optimization experiments, the following conclusion can be drawn:The cocoa wort sample was efficiently extracted under 40℃ for 40 min with 75 μm CAR/PDMS SPME head. The method had linear response within 1-500 mg/L, and the detection limits (S/N＝3) were below 0.023 μg/L. The relative standard deviations were from 3.6%-6.4% and the recoveries were from 95.4% to 102.7%. This method is characterized by rapidly, high sensitivity, good linearity and repeatability for the analysis of cocoa wort samples.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物

通讯作者: 李崎

English

Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction

Corresponding author: 李崎

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物

通讯作者: 李崎

English

Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction

Corresponding author: 李崎

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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