基于工具酶信号放大技术电化学发光法检测凝血酶

引用本文: 朱化雨, 张利, 陈怀成, 闫圣娟. 基于工具酶信号放大技术电化学发光法检测凝血酶[J]. 分析化学, 2012, 40(10): 1549-1554. doi: 10.3724/SP.J.1096.2012.11394 shu

Citation: ZHU Hua-Yu, ZHANG Li, CHEN Huai-Cheng, YAN Sheng-Juan. Electrochemiluminescence Biosensor for Detection of Thrombin Using Enzyme-based Signal Amplification[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1549-1554. doi: 10.3724/SP.J.1096.2012.11394 shu

基于工具酶信号放大技术电化学发光法检测凝血酶

朱化雨 ^{1,
,} ,
张利 ^1, ,
陈怀成 ^1,2, ,
闫圣娟 ^1,2,

通讯作者: 朱化雨

基金项目:
本文系国家自然科学基金(No.21005045) (No.21005045)

山东省科技攻关项目(No.201011002)资助 (No.201011002)

摘要: 利用巯基乙胺将合成的金纳米粒子氨基化;基于纳米粒子负载羧基化的联吡啶钌和巯基DNA制得电化学发光信号探针;采用酶循环信号放大技术,获得大量含新增DNA的溶液来捕获信号探针;以金电极为载体,将巯基DNA自组装到电极表面,依次杂交互补DNA和信号探针,构建电化学发光生物传感器。在优化的条件下,此传感器对凝血酶具有良好的响应,在3.0×10^-13-6.0×10^-11 mol/L范围内,凝血酶的浓度与发光强度呈良好的线性关系,检出限为1.8×10^-13 mol/L(3σ)。采用酶切循环放大技术制备的生物传感器具有灵敏度高,选择性和重现性良好等特点。

关键词:

English

Electrochemiluminescence Biosensor for Detection of Thrombin Using Enzyme-based Signal Amplification

1.
¹ College of Chemistry and Chemical Engineering, LinYi University, Linyi 276005, China;

Corresponding author: 朱化雨

Received Date: 09 December 2011
Fund Project:
本文系国家自然科学基金(No.21005045)

山东省科技攻关项目(No.201011002)资助

Abstract: Amino functionalized gold nanoparticles(GNP) were synthesized in this work. Electrochemiluminescence (ECL) probe was synthesized by covalently linking tris (2,2-bipyridyl) ruthenium (Ⅱ) and self-assembly of thiol DNA5 to this GNP. By using nicking endonuclease signal amplification technology, large amount of newly obtained DNA fragments was obtained to capture ECL probe. Thiol DNA probe was self-assembled on gold electrode surface, and then ECL biosensor was fabricated by sequentially hybridization of complementary DNA (newly obtained DNA fragment) and ECL probe to this modified gold electrode. Under optimized conditions, the ECL intensity of this biosensor exhibited linear relationship with thrombin concentration in the range of 3.0×10^-13-6.0×10^-11 mol/L, and the detection limit was 1.8×10^-13 mol/L (3σ). The biosensor shows good application prospect in the field of biological samples analysis owing to its high sensitivity, good stability and reproducibility.

Key words:

Nanoparticles
/ Nicking endonuclease signal amplification technology
/ Electrogenerated chemiluminescence
/ Thrombin

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于工具酶信号放大技术电化学发光法检测凝血酶

通讯作者: 朱化雨

English

Electrochemiluminescence Biosensor for Detection of Thrombin Using Enzyme-based Signal Amplification

Corresponding author: 朱化雨

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于工具酶信号放大技术电化学发光法检测凝血酶

通讯作者: 朱化雨

English

Electrochemiluminescence Biosensor for Detection of Thrombin Using Enzyme-based Signal Amplification

Corresponding author: 朱化雨

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs