检测禽流感H5亚型病毒的阻抗型免疫研究

引用本文: 颜小飞, 李运涛, 王蓉晖, 林建涵, 温新华, 汪懋华, 安冬, 韩伟静, 俞育德, Yanbin Li. 检测禽流感H5亚型病毒的阻抗型免疫研究[J]. 分析化学, 2012, 40(10): 1507-1513. doi: 10.3724/SP.J.1096.2012.20120 shu

Citation: YAN Xiao-Fei, LI Yun-Tao, WANG Rong-Hui, LIN Jian-Han, WEN Xin-Hua, WANG Mao-Hua, AN Dong, HAN Wei-Jing, YU Yu-De, LI Yan-Bin. An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1507-1513. doi: 10.3724/SP.J.1096.2012.20120 shu

检测禽流感H5亚型病毒的阻抗型免疫研究

颜小飞 ^1, ,
李运涛 ^2, ,
王蓉晖 ^3, ,
林建涵 ^3, ,
温新华 ^1, ,
汪懋华 ^{1,
,} ,
安冬 ^1, ,
韩伟静 ^2, ,
俞育德 ^2, ,
Yanbin Li

通讯作者: 汪懋华

基金项目:
本文系国际科技交流与合作专项(No.2010DFA31000)资助 (No.2010DFA31000)

摘要: 研制了一种可用于H5亚型禽流感病毒快速检测的阻抗型免疫传感器。通过蛋白A将H5N1表面抗原血凝素(HA)的单克隆抗体固定于金叉指阵列微电极表面,并与待测溶液中的目标抗原H5N1进行免疫反应。在[Fe(CN)₆]^3-/4-溶液中进行电化学阻抗谱扫描,表征电极的表面修饰及抗原捕获过程。当H5N1病毒浓度在2¹-2⁶ HA unit/50 μL范围时,其浓度的对数值与叉指阵列微电极的电子传递阻抗的变化值呈线性关系,相关系数为0.9885;检出限为2⁰ HA unit/50 μL,检测时间为1 h。此传感器特异性好,灵敏度高,可以重复使用,在病原微生物快速检测领域具有良好的应用前景。

关键词:

English

An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus

1.
¹ Key Laboratory on Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China;

Corresponding author: 汪懋华

Received Date: 14 February 2012
Fund Project:
本文系国际科技交流与合作专项(No.2010DFA31000)资助

Abstract: An impedance immunosensor was developed for the rapid detection of H5 subtype avian influenza virus (AIV). Monoclonal antibodies against AIV H5N1 surface antigen hemagglutinin (HA) were immobilized on the surface of gold interdigitated array microelectrodes through protein A for capturing AIV H5N1 in sample solutions. Electrochemical impedance spectroscopy in the presence of [Fe(CN)₆]^3-/4- as a redox probe was used to describe the surface modification of microelectrodes and the binding of viruses. A linear relationship between the logarithmic value of concentration of AIV H5N1 and the change of electron transfer resistance was found in the concentration range of 2¹-2⁶ HA unit per 50 μL, and its correlation coefficient was 0.9885. The detection limit was 2⁰ HA unit per 50 μL, and the detection time was 1 h. This immunosensor could be used repeatedly with good specificity and high sensitivity, and it is promising for rapid detection of pathogenic microorganisms.

Key words:

Impedance immunosensor
/ Avian influenza virus
/ Electrochemical impedance spectroscopy
/ Interdigitated array microelectrodes

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

检测禽流感H5亚型病毒的阻抗型免疫研究

通讯作者: 汪懋华

English

An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus

Corresponding author: 汪懋华

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

检测禽流感H5亚型病毒的阻抗型免疫研究

通讯作者: 汪懋华

English

An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus

Corresponding author: 汪懋华

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs