聚多巴胺包埋G-四联体/血红素DNA酶制备过氧化氢生物传感器

引用本文: 高艾, 王玉茹, 何锡文, 尹学博. 聚多巴胺包埋G-四联体/血红素DNA酶制备过氧化氢生物传感器[J]. 分析化学, 2012, 40(10): 1471-1476. doi: 10.3724/SP.J.1096.2012.20095 shu

Citation: GAO Ai, WANG Yu-Ru, HE Xi-Wen, YIN Xue-Bo. An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1471-1476. doi: 10.3724/SP.J.1096.2012.20095 shu

聚多巴胺包埋G-四联体/血红素DNA酶制备过氧化氢生物传感器

通讯作者: 尹学博

基金项目:
本文系国家自然科学基金(No.21075068) (No.21075068)

973 项目(No.2011CB707703) (No.2011CB707703)

天津市自然科学基金(No.11JCZDJC22200) (No.11JCZDJC22200)

电分析化学国家重点实验室开放基金和高等学校基本科研业务费资助

摘要: 利用多巴胺的氧化自聚实现对G-四联体/血红素DNA酶的包埋,成功构建了H₂O₂电化学生物传感器。DNA和血红素混合得到G-四联体/血红素复合物;DNA酶物理吸附在玻碳电极上后,将10 μL 5 g/L多巴胺的磷酸盐缓冲液(pH 8.0)滴在表面,空气中的氧气氧化多巴胺形成聚多巴胺膜,实现DNA酶的固定。考察了不同DNA序列对传感器性能的影响,表明电化学与光学传感过程具有不同序列响应。此传感器对H₂O₂的检出限为2.2 μmol/L; 线性范围为0.01-1.5 mmol/L。本研究证实了利用聚多巴胺固定酶和用DNA酶代替天然酶构筑传感器的可行性。

关键词:

English

An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

1.
State Key Laboratory of Medicinal Chemical Biology and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China

Corresponding author: 尹学博

Received Date: 08 February 2012
Fund Project:
本文系国家自然科学基金(No.21075068)

973 项目(No.2011CB707703)

天津市自然科学基金(No.11JCZDJC22200)

电分析化学国家重点实验室开放基金和高等学校基本科研业务费资助

Abstract: The complex of G-quadruplex-hemin, also named peroxidase-mimicking DNAzyme, shows a catalytic activity towards hydrogen peroxide, which is rarely used as a catalyst in the assembly of electrochemical biosensors. Meanwhile, it is of significance to develop novel method in immobilizing DNA for the purpose of fabrication of DNA-based biosensors. Dopamine can form polydopamine film via self-polymerization. In this study, an electrochemical hydrogen peroxide biosensor was successfully constructed using polydopamine-entrapped G-quadruplex-hemin DNAzyme. The mixture of DNA and hemin achieved the formation of G-quadruplex-hemin complex. After the physical adsorption of DNAzyme on the glassy carbon electrode, a droplet containing 5 g/L dopamine in PBS (pH 8.0) was cast onto the surface. Ambient oxygen in the air oxidized dopamine for the formation of polydopamine to retain DNAzyme. The influence of DNA sequence to electrochemistry was investigated, showing a different phenomenon to optical sensors. The proposed biosensor was sensitive to hydrogen peroxide with the linear range spans the concentration from 10 μmol/L to 1.5 mmol/L and the limit of detection of 2.2 μmol/L. The results demonstrated the possibility of enzyme immobilization on the electrode through polydopamine and the substitution of G-quadruplex-hemin DNAzyme for natural enzyme.

Key words:

1. [1]
  1 Tan X, Zhang J, Tan S, Zhao D, Huang Z, Mi Y, Huang Z. Electroanal., 2009, 21(13): 1514-15201 Tan X, Zhang J, Tan S, Zhao D, Huang Z, Mi Y, Huang Z. Electroanal., 2009, 21(13): 1514-1520
2. [2]
  2 Zhou K, Zhu Y, Yang X, Luo J, Li C, Luan S. Electrochim. Acta, 2010, 55(9): 3055-30602 Zhou K, Zhu Y, Yang X, Luo J, Li C, Luan S. Electrochim. Acta, 2010, 55(9): 3055-3060
3. [3]
  3 ZHENG Long-Zhen, LI Yin-Di, XIONG Le-Yan, LIU Wen, LIU Qiang, HAN Kui, YANG Shao-Ming, XIA Jian. Chinese J. Anal. Chem., 2012, 40(1): 72-763 ZHENG Long-Zhen, LI Yin-Di, XIONG Le-Yan, LIU Wen, LIU Qiang, HAN Kui, YANG Shao-Ming, XIA Jian. Chinese J. Anal. Chem., 2012, 40(1): 72-76
4. [4]
  郑龙珍, 李引弟, 熊乐艳, 刘文, 刘强, 韩奎, 杨绍明, 夏坚. 分析化学, 2012, 40(1): 72-76郑龙珍, 李引弟, 熊乐艳, 刘文, 刘强, 韩奎, 杨绍明, 夏坚. 分析化学, 2012, 40(1): 72-76
5. [5]
  4 LIU Yan, NIU Wei-Fen, XU Lan. Chinese J. Anal. Chem., 2011, 39(11): 1676-16814 LIU Yan, NIU Wei-Fen, XU Lan. Chinese J. Anal. Chem., 2011, 39(11): 1676-1681
6. [6]
  刘艳,牛卫芬,徐岚. 分析化学, 2011, 39(11): 1676-1681刘艳,牛卫芬,徐岚. 分析化学, 2011, 39(11): 1676-1681
7. [7]
  5 You J-M, Jeong Y N, Ahmed M S, Kim S K, Choi H C, Jeon S. Biosens. Bioelectron., 2011, 26(5): 2287-22915 You J-M, Jeong Y N, Ahmed M S, Kim S K, Choi H C, Jeon S. Biosens. Bioelectron., 2011, 26(5): 2287-2291
8. [8]
  6 Liu H, Wen M, Zhang F, Liu D, Tian Y. Anal. Methods, 2010, 2(2): 143-1486 Liu H, Wen M, Zhang F, Liu D, Tian Y. Anal. Methods, 2010, 2(2): 143-148
9. [9]
  7 Guascito M R, Chirizzi D, Malitesta C, Mazzotta E, Siciliano M, Siciliano T, Tepore A, Turco A. Biosens. Bioe-lectron., 2011, 26(8): 3562-35697 Guascito M R, Chirizzi D, Malitesta C, Mazzotta E, Siciliano M, Siciliano T, Tepore A, Turco A. Biosens. Bioe-lectron., 2011, 26(8): 3562-3569
10. [10]
  8 Travascio P, Li Y, Sen D. Chem. Biol., 1998, 5(9): 505-5178 Travascio P, Li Y, Sen D. Chem. Biol., 1998, 5(9): 505-517
11. [11]
  9 Li T, Shi L, Wang E, Dong S. Chem. Eur. J., 2009, 15(4): 1036-10429 Li T, Shi L, Wang E, Dong S. Chem. Eur. J., 2009, 15(4): 1036-1042
12. [12]
  10 XU Jing, KONG De-Ming. Chinese J. Anal. Chem., 2012, 40(3): 347-35310 XU Jing, KONG De-Ming. Chinese J. Anal. Chem., 2012, 40(3): 347-353
13. [13]
  徐静, 孔德明. 分析化学, 2012, 40(3): 347-353徐静, 孔德明. 分析化学, 2012, 40(3): 347-353
14. [14]
  11 Qiu B, Zheng Z Z, Lu Y J, Lin Z Y, Wong K Y, Chen G N. Chem. Commun., 2011, 47(5): 1437-143911 Qiu B, Zheng Z Z, Lu Y J, Lin Z Y, Wong K Y, Chen G N. Chem. Commun., 2011, 47(5): 1437-1439
15. [15]
  12 Pavlov V, Xiao Y, Gill R, Dishon A, Kotler M, Willner I. Anal. Chem., 2004, 76(7): 2152-215612 Pavlov V, Xiao Y, Gill R, Dishon A, Kotler M, Willner I. Anal. Chem., 2004, 76(7): 2152-2156
16. [16]
  13 Lee H, Dellatore S M, Miller W M, Messersmith P B. Science, 2007, 318(5849): 426-43013 Lee H, Dellatore S M, Miller W M, Messersmith P B. Science, 2007, 318(5849): 426-430
17. [17]
  14 Yin X B, Liu D Y. J. Chromatogr. A, 2008, 1212(1-2): 130-13614 Yin X B, Liu D Y. J. Chromatogr. A, 2008, 1212(1-2): 130-136
18. [18]
  15 Xing B, Yin X B. PLoS One, 2009, 4(7): e645115 Xing B, Yin X B. PLoS One, 2009, 4(7): e6451
19. [19]
  16 Sundquist W I, Klug A. Nature, 1989, 342(6251): 825-82916 Sundquist W I, Klug A. Nature, 1989, 342(6251): 825-829
20. [20]
  17 Li T, Li B, Dong S. Anal. Bioanal. Chem., 2007, 389(3): 887-89317 Li T, Li B, Dong S. Anal. Bioanal. Chem., 2007, 389(3): 887-893
21. [21]
  18 Li T, Dong S, Wang E. Chem. Asian J., 2009, 4(6): 918-92218 Li T, Dong S, Wang E. Chem. Asian J., 2009, 4(6): 918-922
22. [22]
  19 Nakayama S, Wang J, Sintim H O. Chem. Eur. J., 2011, 17(20): 5691-569819 Nakayama S, Wang J, Sintim H O. Chem. Eur. J., 2011, 17(20): 5691-5698
23. [23]
  20 Kong D M, Yang W, Wu J, Li C X, Shen H X. Analyst, 2010, 135(2): 321-32620 Kong D M, Yang W, Wu J, Li C X, Shen H X. Analyst, 2010, 135(2): 321-326

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 894
HTML全文浏览量: 77

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

聚多巴胺包埋G-四联体/血红素DNA酶制备过氧化氢生物传感器

通讯作者: 尹学博

English

An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

Corresponding author: 尹学博

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

聚多巴胺包埋G-四联体/血红素DNA酶制备过氧化氢生物传感器

通讯作者: 尹学博

English

An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

Corresponding author: 尹学博

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs