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Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor

Mohammad Hossein Mashhadizadeh ,  Rasoul Pourtaghavi Talemi

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Citation:  Mohammad Hossein Mashhadizadeh, Rasoul Pourtaghavi Talemi. Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor[J]. Chinese Chemical Letters, 2015, 26(1): 160-166. doi: 10.1016/j.cclet.2014.09.004 shu

Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor

    • 通讯作者: Rasoul Pourtaghavi Talemi,
摘要: An effective procedure for constructing a DNA biosensor is developed based on covalent immobilization of NH2 labeled, single strand DNA (NH2-ssDNA) onto a self-assembled diazo-thiourea and gold nanoparticles modified Au electrode (diazo-thiourea/GNM/Au). Gold nano-particles expand the electrode surface area and increase the amount of immobilized thiourea and single stranded DNA (ssDNA) onto the electrode surface. Diazo-thiourea film provides a surface with high conductibility for electron transfer and a bed for the covalent coupling of NH2-ssDNA onto the electrode surface. The immobilization and hybridization of the probe DNA on the modified electrode is studied by differential pulse voltammetry (DPV) using methylene blue (MB) as a well-known electrochemical hybridization indicator. The linear range for the determination of complementary target ssDNA is from 9.5(±0.1)×10-13 mol/L to 1.2(±0.2)×10-9 mol/L with a detection limit of 1.2(±0.1)×10-13mol/L.

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  • 发布日期:  2014-09-16
  • 收稿日期:  2014-05-26
  • 网络出版日期:  2014-08-29
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