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Chemical compositions and effects on chemiluminescence of AMs in vitro of chalk dusts

Yue-Xia Zhang ,  Zhen-Hua Yang ,  Quan-Xi Zhang ,  Rui-Jin Li ,  Hong Geng ,  Chuan Dong

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Citation:  Yue-Xia Zhang, Zhen-Hua Yang, Quan-Xi Zhang, Rui-Jin Li, Hong Geng, Chuan Dong. Chemical compositions and effects on chemiluminescence of AMs in vitro of chalk dusts[J]. Chinese Chemical Letters, 2015, 26(1): 157-159. doi: 10.1016/j.cclet.2014.08.004 shu

Chemical compositions and effects on chemiluminescence of AMs in vitro of chalk dusts

    • 通讯作者: Hong Geng,
    Chuan Dong,
摘要: The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters (PM2.5) on rat alveolar macrophages (AMs) in vitro. Morphologies and element concentrations of chalk particles were analyzed using quantitative energydispersive electron probe X-ray microanalysis (ED-EPMA). The oxidative response of AMs exposed to chalk PM2.5 was measured by luminol-dependent chemiluminescence (CL). The results showed that (1) Chalk dust was mainly composed of gypsum (CaSO4), calcite (CaCO3)/dolomite (CaMg(CO3)2), and organic adhesives; (2) Fine chalk particles induced the AM production of CL, which was inhibited by about 90% by diphenyleneiodonium chloride (DPI). Based on these results, we showed that cytotoxicity of chalk PM2.5 may be related to the reactive oxygen species (ROS) generation.

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    1. [1] P.C. Zeidler, A. Hubbs, L. Battelli, V. Castranova, Role of inducible nitric oxide synthase-derived nitric oxide in silica-induced pulmonary inflammation and fibrosis, J. Toxicol. Environ. Health A 67 (2004) 1001-1026.[1] P.C. Zeidler, A. Hubbs, L. Battelli, V. Castranova, Role of inducible nitric oxide synthase-derived nitric oxide in silica-induced pulmonary inflammation and fibrosis, J. Toxicol. Environ. Health A 67 (2004) 1001-1026.

    2. [2] R.J. Li, X.J. Kou, H. Geng, C. Dong, Z.W. Cai, Pollution characteristics of ambient PM2.5-bound PAHs and NPAHs in a typical winter time period in Taiyuan, Chin. Chem. Lett. 25 (2014) 663-666.[2] R.J. Li, X.J. Kou, H. Geng, C. Dong, Z.W. Cai, Pollution characteristics of ambient PM2.5-bound PAHs and NPAHs in a typical winter time period in Taiyuan, Chin. Chem. Lett. 25 (2014) 663-666.

    3. [3] E. Bastonini, L. Verdone, S. Morrone, et al., Transcriptional modulation of a human monocytic cell line exposed to PM10 from an urban area, Environ. Res. 111 (2011) 765-774.[3] E. Bastonini, L. Verdone, S. Morrone, et al., Transcriptional modulation of a human monocytic cell line exposed to PM10 from an urban area, Environ. Res. 111 (2011) 765-774.

    4. [4] D. Majumdar, S.P.M. Prince William, Chalk dust fall during classroom teaching: particle size distribution and morphological characteristics, Environ. Monit. Assess. 148 (2009) 343-351.[4] D. Majumdar, S.P.M. Prince William, Chalk dust fall during classroom teaching: particle size distribution and morphological characteristics, Environ. Monit. Assess. 148 (2009) 343-351.

    5. [5] S. Weichenthal, A. Dufresne, C. Infante-Rivard, L. Joseph, Characterizing and predicting ultrafine particle counts in Canadian classrooms during the winter months: model development and evaluation, Environ. Res. 106 (2008) 349-360.[5] S. Weichenthal, A. Dufresne, C. Infante-Rivard, L. Joseph, Characterizing and predicting ultrafine particle counts in Canadian classrooms during the winter months: model development and evaluation, Environ. Res. 106 (2008) 349-360.

    6. [6] H. Geng, J.Y. Ryu, S. Maskey, H.J. Jung, C.U. Ro, Characterisation of individual aerosol particles collected during a haze episode in Incheon, Korea using the quantitative ED-EPMA technique, Atmos. Chem. Phys. 11 (2011) 1327-1337.[6] H. Geng, J.Y. Ryu, S. Maskey, H.J. Jung, C.U. Ro, Characterisation of individual aerosol particles collected during a haze episode in Incheon, Korea using the quantitative ED-EPMA technique, Atmos. Chem. Phys. 11 (2011) 1327-1337.

    7. [7] Q.N. Myrvik, E.S. Leake, B. Fariss, Lysozyme content of alveolar and peritoneal macrophages from the rabbit, J. Immunol. 86 (1961) 133-136.[7] Q.N. Myrvik, E.S. Leake, B. Fariss, Lysozyme content of alveolar and peritoneal macrophages from the rabbit, J. Immunol. 86 (1961) 133-136.

    8. [8] H. Geng, Z.Q. Meng, Inhibition of superoxide dismutase, vitamin C and glutathione on chemiluminescence produced by luminol and the mixture of sulfite and bisulfate, Spectrochim. Acta A 64 (2006) 87-92.[8] H. Geng, Z.Q. Meng, Inhibition of superoxide dismutase, vitamin C and glutathione on chemiluminescence produced by luminol and the mixture of sulfite and bisulfate, Spectrochim. Acta A 64 (2006) 87-92.

    9. [9] T.T. Chen, Y.H. Hu, Y. Cen, X. Chu, Y. Lu, A dual-emission fluorescent nanocomplex of gold-cluster-decorated silica particles for live cell imaging of highly reactive oxygen species, J. Am. Chem. Soc. 135 (2013) 11595-11602.[9] T.T. Chen, Y.H. Hu, Y. Cen, X. Chu, Y. Lu, A dual-emission fluorescent nanocomplex of gold-cluster-decorated silica particles for live cell imaging of highly reactive oxygen species, J. Am. Chem. Soc. 135 (2013) 11595-11602.

    10. [10] C.U. Ro, J. Osá n, I. Szalóki, et al., A Monte Carlo program for quantitative electron-induced X-ray analysis of individual particles, Anal. Chem. 75 (2003) 851-859.[10] C.U. Ro, J. Osá n, I. Szalóki, et al., A Monte Carlo program for quantitative electron-induced X-ray analysis of individual particles, Anal. Chem. 75 (2003) 851-859.

    11. [11] A. Clouter, C.E. Houghton, L.R. Hibbs, et al., Effect of inhalation of low doses of crocidolite and fibrous gypsum on the glutathione concentration and g-glutamyl transpeptidase activity in macrophages and bronchoalveolar lavage fluid, Inhal. Toxicol. 10 (1998) 3-14.[11] A. Clouter, C.E. Houghton, L.R. Hibbs, et al., Effect of inhalation of low doses of crocidolite and fibrous gypsum on the glutathione concentration and g-glutamyl transpeptidase activity in macrophages and bronchoalveolar lavage fluid, Inhal. Toxicol. 10 (1998) 3-14.

    12. [12] E.F.M. Wouters, T.H.J.M. Jorna, M. Westenend, Respiratory effects of coal dust exposure: clinical effects and diagnosis, Exp. Lung Res. 20 (1994) 385-394.[12] E.F.M. Wouters, T.H.J.M. Jorna, M. Westenend, Respiratory effects of coal dust exposure: clinical effects and diagnosis, Exp. Lung Res. 20 (1994) 385-394.

    13. [13] F. Crummy, I. Carl, C.H.S. Cameron, et al., A possible case of pneumoconiosis in a limestone quarry worker, Occup. Med. 54 (2004) 497-499.[13] F. Crummy, I. Carl, C.H.S. Cameron, et al., A possible case of pneumoconiosis in a limestone quarry worker, Occup. Med. 54 (2004) 497-499.

    14. [14] N.A. Paterson, D.J. McIver, S. Schurch, The effect of leukotrienes on porcine alveolar macrophage function, Prostaglandins Leukot. Med. 25 (1986) 147-161.[14] N.A. Paterson, D.J. McIver, S. Schurch, The effect of leukotrienes on porcine alveolar macrophage function, Prostaglandins Leukot. Med. 25 (1986) 147-161.

    15. [15] Z. Zhang, H.M. Shen, Q.F. Zhang, C.N. Ong, Involvement of oxidative stress in crystalline silica induced cytotoxicity and genotoxicity in rat alveolar macrophages, Environ. Res. 82 (2000) 245-252.[15] Z. Zhang, H.M. Shen, Q.F. Zhang, C.N. Ong, Involvement of oxidative stress in crystalline silica induced cytotoxicity and genotoxicity in rat alveolar macrophages, Environ. Res. 82 (2000) 245-252.

    16. [16] A.M. Scherbart, J. Langer, A. Bushmelev, et al., Contrasting macrophage activation by fine and ultrafine titanium dioxide particles is associated with different uptake mechanisms, Part. Fibre Toxicol. 8 (2011) 31-49.[16] A.M. Scherbart, J. Langer, A. Bushmelev, et al., Contrasting macrophage activation by fine and ultrafine titanium dioxide particles is associated with different uptake mechanisms, Part. Fibre Toxicol. 8 (2011) 31-49.

    17. [17] B. Halliwell, J.M.C. Gutteridge, Free Radicals in Biology and Medicine, Claredon Press, Oxford, 1989.[17] B. Halliwell, J.M.C. Gutteridge, Free Radicals in Biology and Medicine, Claredon Press, Oxford, 1989.

    18. [18] D. Breznan, P. Goegan, V. Chauhan, et al., Respiratory burst in alveolar macrophages exposed to urban particles is not a predictor of cytotoxicity, Toxicol. In Vitro 27 (2013) 1287-1297.[18] D. Breznan, P. Goegan, V. Chauhan, et al., Respiratory burst in alveolar macrophages exposed to urban particles is not a predictor of cytotoxicity, Toxicol. In Vitro 27 (2013) 1287-1297.

    19. [19] Y. Li, M.A. Trush, Diphenyleneiodonium, an NAD(P)H oxidase inhibitor, also potently inhibits mitochondrial reactive oxygen species production, Biochem. Biophys. Res. Commun. 253 (1998) 295-299.[19] Y. Li, M.A. Trush, Diphenyleneiodonium, an NAD(P)H oxidase inhibitor, also potently inhibits mitochondrial reactive oxygen species production, Biochem. Biophys. Res. Commun. 253 (1998) 295-299.

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  • 发布日期:  2014-09-01
  • 收稿日期:  2014-05-05
  • 网络出版日期:  2014-08-25
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