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生物小分子钒配合物抑制蛋白酪氨酸磷酸酶活性研究

李鹏宇 ,  卢丽萍

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引用本文: 李鹏宇, 卢丽萍. 生物小分子钒配合物抑制蛋白酪氨酸磷酸酶活性研究[J]. 无机化学学报, 2013, 29(9): 1830-1834. doi: 10.3969/j.issn.1001-4861.2013.00.229 shu
Citation:  LI Peng-Yu, LU Li-Ping. PTPs Inhibition by Some Oxovanadium Complexes with Bioligands[J]. Chinese Journal of Inorganic Chemistry, 2013, 29(9): 1830-1834. doi: 10.3969/j.issn.1001-4861.2013.00.229 shu

生物小分子钒配合物抑制蛋白酪氨酸磷酸酶活性研究

  • 基金项目:

    国家自然科学基金(No.21171109) (No.21171109)

    山西省自然科学基金(2011011009-1) (2011011009-1)

    山西省回国留学人员科研课题(2012-004)资助项目。 (2012-004)

摘要: 钒化合物治疗糖尿病机理研究表明其与蛋白酪氨酸磷酸酶的酶活抑制有一定关系。本文分别研究了生物小分子配体与氧钒离子在20:1配比条件下形成的生物小分子钒配合物及其对蛋白酪氨酸磷酸酶的抑制作用和选择性。结果表明氨基酸与氧钒离子配合形成2:1的配合物,而抗坏血酸及多羧酸与氧钒离子配合形成1:1的配合物。它们对蛋白酪氨酸磷酸酶抑制作用显示,大部分生物小分子氧钒配合物对PTP1B表现强烈的抑制作用,IC50值在0.12~0.63 μmol·L-1之间。化合物[VO(Phe)2]表现最强的抑制作用,IC50值为0.07 μmol·L-1。而[VO(Arg)2]、[VO(Oxalate)]、[VO(Nitrilotriacetate)]和[VO(Citrate)]则呈现较弱的抑制,IC50值分别为1.05、1.41、9.90和21.5 μmol·L-1。对PTP1B, TCPTP, HePTP以及SHP-1的抑制作用表明配体的结构不仅影响氧钒配合物对蛋白酪氨酸磷酸酶的抑制效率同时也影响其选择性。

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    1. [1] Shechter Y, Karlish S J D. Nature, 1980,284:556-558[1] Shechter Y, Karlish S J D. Nature, 1980,284:556-558

    2. [2] Thompson K H, McNeill J H, Orvig C. Chem. Rev., 1999, 99:2561-2571[2] Thompson K H, McNeill J H, Orvig C. Chem. Rev., 1999, 99:2561-2571

    3. [3] Thompson K H, Lichter J, LeBel C, et al. J. Inorg. Biochem., 2009,103:554-558[3] Thompson K H, Lichter J, LeBel C, et al. J. Inorg. Biochem., 2009,103:554-558

    4. [4] Thompson K H, Orvig C. J. Inorg. Biochem., 2006,100:1925-1935[4] Thompson K H, Orvig C. J. Inorg. Biochem., 2006,100:1925-1935

    5. [5] Crans D C, Smee J J, Gaidamauskas E, et al. Chem. Rev., 2004,104:849-902[5] Crans D C, Smee J J, Gaidamauskas E, et al. Chem. Rev., 2004,104:849-902

    6. [6] Elchebly M. Science, 1999,283:1544-1548[6] Elchebly M. Science, 1999,283:1544-1548

    7. [7] Zhang S, Zhang Z Y. Drug Discovery Today, 2007,12:373-381[7] Zhang S, Zhang Z Y. Drug Discovery Today, 2007,12:373-381

    8. [8] Thareja S, Aggarwal S, Bhardwaj T R, et al. Med. Res. Rev., 2012,32:459-517[8] Thareja S, Aggarwal S, Bhardwaj T R, et al. Med. Res. Rev., 2012,32:459-517

    9. [9] Yuan C, Lu L, Gao X, et al. J. Biol. Inorg. Chem., 2009,14: 841-851[9] Yuan C, Lu L, Gao X, et al. J. Biol. Inorg. Chem., 2009,14: 841-851

    10. [10] Lu L, Wang S, Zhu M, et al. Biometals, 2010,23:1139-1147[10] Lu L, Wang S, Zhu M, et al. Biometals, 2010,23:1139-1147

    11. [11] Yuan C, Lu L, Wu Y, et al. J. Inorg. Biochem., 2010,104: 978-986[11] Yuan C, Lu L, Wu Y, et al. J. Inorg. Biochem., 2010,104: 978-986

    12. [12] Lu L, Yue J, Yuan C, et al. J. Inorg. Biochem., 2011,105: 1323-1328[12] Lu L, Yue J, Yuan C, et al. J. Inorg. Biochem., 2011,105: 1323-1328

    13. [13] Han H, Lu L, Wang Q, et al. Dalton Trans., 2012,41:11116-11124[13] Han H, Lu L, Wang Q, et al. Dalton Trans., 2012,41:11116-11124

    14. [14] Lu L, Gao X, Zhu M, et al. Biometals, 2012,25:599-610[14] Lu L, Gao X, Zhu M, et al. Biometals, 2012,25:599-610

    15. [15] GAO Xiao-Li(高晓丽), LU Li-Ping(卢丽萍), ZHU Miao-Li (朱苗力), et al. Acta Chim. Sin.(Huaxue Xuebao), 2009,67: 929-936[15] GAO Xiao-Li(高晓丽), LU Li-Ping(卢丽萍), ZHU Miao-Li (朱苗力), et al. Acta Chim. Sin.(Huaxue Xuebao), 2009,67: 929-936

    16. [16] Lu L, Zhu M. Anti-Cancer Agents Med. Chem., 2011,11:164-171[16] Lu L, Zhu M. Anti-Cancer Agents Med. Chem., 2011,11:164-171

    17. [17] ZHANG Heng-Qiang(张恒强),ZHANG Qi-Ying(张其颖),LI Jia(李佳), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2012,33:243-250[17] ZHANG Heng-Qiang(张恒强),ZHANG Qi-Ying(张其颖),LI Jia(李佳), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2012,33:243-250

    18. [18] McLauchlan C C, Hooker J D, Jones M A, et al. J. Inorg. Biochem., 2010,104:274-281[18] McLauchlan C C, Hooker J D, Jones M A, et al. J. Inorg. Biochem., 2010,104:274-281

    19. [19] Ma L, Lu L, Zhu M, et al. Dalton Ttrans., 2011,40:6532-6540[19] Ma L, Lu L, Zhu M, et al. Dalton Ttrans., 2011,40:6532-6540

    20. [20] Qi Y, Zhao R, Cao H, et al. J. Cell. Biochem., 2002,86:79-89[20] Qi Y, Zhao R, Cao H, et al. J. Cell. Biochem., 2002,86:79-89

    21. [21] Li W, Zhuang Y, Li H, et al. Chem. Res. Chin. Univ., 2008, 24:592-596[21] Li W, Zhuang Y, Li H, et al. Chem. Res. Chin. Univ., 2008, 24:592-596

    22. [22] Zhu Z, Sun M, Zhang X, et al. Chem. Res. Chin. Univ., 2007, 23:289-296[22] Zhu Z, Sun M, Zhang X, et al. Chem. Res. Chin. Univ., 2007, 23:289-296

    23. [23] Pessoa J C, Boas L F V, Gillard R D. Polyhedron, 1989,8: 1173-1199[23] Pessoa J C, Boas L F V, Gillard R D. Polyhedron, 1989,8: 1173-1199

    24. [24] Pessoa J C, Boas L F V, Gillard R D, et al. Polyhedron, 1988, 7:1245-1262[24] Pessoa J C, Boas L F V, Gillard R D, et al. Polyhedron, 1988, 7:1245-1262

    25. [25] Pessoa J C, Boas L F V, Gillard R D. Polyhedron, 1989,8: 1745-1747[25] Pessoa J C, Boas L F V, Gillard R D. Polyhedron, 1989,8: 1745-1747

    26. [26] Costa Pessoa J, Luz S M, Cavaco I, et al. Polyhedron, 1994, 13:3177-3198[26] Costa Pessoa J, Luz S M, Cavaco I, et al. Polyhedron, 1994, 13:3177-3198

    27. [27] Pessoa J C, Marques R L, Boas L F V, et al. Polyhedron, 1990,9:81-98[27] Pessoa J C, Marques R L, Boas L F V, et al. Polyhedron, 1990,9:81-98

    28. [28] Pessoa J C, Antunest J L, Boas L F V et al. Polyhedron, 1990,9:2101-2125[28] Pessoa J C, Antunest J L, Boas L F V et al. Polyhedron, 1990,9:2101-2125

    29. [29] Pessoa J C, Antunest J L, Boas L F V, et al. Polyhedron, 1992,11:1449-1461[29] Pessoa J C, Antunest J L, Boas L F V, et al. Polyhedron, 1992,11:1449-1461

    30. [30] Lodyga-Chruscinska E, Sanna D, Garribba E, et al. Dalton Trans., 2008,4903-4916[30] Lodyga-Chruscinska E, Sanna D, Garribba E, et al. Dalton Trans., 2008,4903-4916

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  • 收稿日期:  2013-01-14
  • 网络出版日期:  2013-03-22
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