溶菌酶蛋白在聚合物防污膜表面的吸附

引用本文: 胡立梅, 蔺存国, 王利, 苑世领. 溶菌酶蛋白在聚合物防污膜表面的吸附[J]. 物理化学学报, 2014, 30(11): 2149-2156. doi: 10.3866/PKU.WHXB201409021 shu

Citation: HU Li-Mei, LIN Cun-Guo, WANG Li, YUAN Shi-Ling. Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface[J]. Acta Physico-Chimica Sinica, 2014, 30(11): 2149-2156. doi: 10.3866/PKU.WHXB201409021 shu

溶菌酶蛋白在聚合物防污膜表面的吸附

基金项目:
国家重点基础研究发展规划项目(973) (2014CB643305)资助 (973) (2014CB643305)

摘要:

采用分子动力学模拟方法比较了溶菌酶蛋白在两种典型聚合物防污材料聚乙二醇(PEG)和聚二甲基硅氧烷(PDMS)表面的吸附行为, 在微观上探讨了聚合物膜表面性质对蛋白质吸附的影响. 根据蛋白质与聚合物膜之间的相互作用、能量变化及表面水化层分子的动力学行为, 解释了PEG防污涂层相对于PDMS表面具有更佳防污效果的原因: (1) 相比PDMS涂层, 蛋白质与PEG涂层的结合能量较低, 使其结合更加疏松; (2) 蛋白质吸附到材料表面要克服表面水化层分子引起的能障, PEG表面与水分子之间结合紧密, 结合水难于脱附, 造成蛋白质在其表面的吸附需要克服更高的能量, 不利于蛋白质的吸附.

关键词:

English

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

1.
1. Key Laboratory of Colloid and Interface Chemistry, Shandong University, Jinan 250100, P. R. China;
2. State Key Laboratory for Marine Corrosion and Protection, Luoyang Ship Material Research Institute LSMRI, Qingdao 266101, Shandong Province, P. R. China
Received Date: 31 July 2014
Available Online: 30 October 2014
Fund Project:
国家重点基础研究发展规划项目(973) (2014CB643305)资助

Abstract:

Molecular dynamics simulations were used to compare the adsorption behavior of lysozyme on two typical antifouling polymer materials: poly(ethylene) glycol (PEG) and poly(dimethylsiloxane) (PDMS). The influence of the surface properties of the polymer films on protein adsorption is discussed at the microscale. Based on the interactions, energy changes between the protein and polymer films, and dynamical behavior of the hydration molecules near the polymer film, the reasons why the PEG antifouling coating has a better antifouling effect than the PDMS surface were determined as follows. (1) The lower binding energy between the protein and the PEG coating than between the protein and the PDMS coating makes the protein adsorb weaker on the PEG coating than on the PDMS coating. (2) The protein would adsorb on the film surface when overcoming the energy barrier caused by the hydration layer. Molecular water adsorbs on the PEG surface stronger than on the PDMS surface, and is difficult to desorb. Therefore, the protein needs to overcome a higher energy barrier to adsorb to the PEG surface than to the PDMS surface, and thus it is more difficult for protein to absorb on the PEG surface than on the PDMS surface.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

溶菌酶蛋白在聚合物防污膜表面的吸附

English

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

溶菌酶蛋白在聚合物防污膜表面的吸附

English

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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