双光子敏化Eu<sup>3+</sup>高效发光活细胞成像纳米生物探针

引用本文: 符小艺, 邵光胜, 韩荣成, 马严, 薛富民, 杨帆, 付立民, 张建平, 王远. 双光子敏化Eu³⁺高效发光活细胞成像纳米生物探针[J]. 物理化学学报, 2012, 28(10): 2480-2486. doi: 10.3866/PKU.WHXB201208161 shu

Citation: FU Xiao-Yi, SHAO Guang-Sheng, HAN Rong-Cheng, MA Yan, XUE Fu-Min, YANG Fan, FU Li-Min, ZHANG Jian-Ping, WANG Yuan. Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging[J]. Acta Physico-Chimica Sinica, 2012, 28(10): 2480-2486. doi: 10.3866/PKU.WHXB201208161 shu

双光子敏化Eu³⁺高效发光活细胞成像纳米生物探针

符小艺 ^1,2, ,
邵光胜 ^1, ,
韩荣成 ^1, ,
马严 ^1, ,
薛富民 ^1, ,
杨帆 ^3, ,
付立民 ^3, ,
张建平 ^3, ,
王远 ^1,

基金项目:
国家自然科学基金(21073002, 21133001, 21227803, 51121091) (21073002, 21133001, 21227803, 51121091)

国家重点基础研究专项经费(2011CB808702)资助 (2011CB808702)

摘要:

将Eu(tta)₃dpbt (dpbt: 2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine; tta:thenoyltrifluoroacetonato)包埋在甲基丙烯酸甲酯-苯乙烯共聚物、正辛基三甲氧基硅及其水解缩合产物组成的杂化基质中, 制备了Eu(tta)₃dpbt 质量分数为40%的荧光纳米粒子, 其平均粒径为45 nm. 所制备的发光纳米粒子在水中分散稳定性高、光稳定性好、细胞毒性低、长波敏化Eu³⁺发光性能优良, 适宜作为生物分析的发光标记物. 所制备的发光纳米粒子的可见区激发峰位于415 nm, 激发峰尾部延展至475 nm, 其发光量子产率为0.31(λ_ex=415 nm, T=23 ℃), 最大双光子激发作用截面为5.0×10⁵ GM (λ_ex=830 nm, 1 GM=10^-50 cm⁴·s·photo^-1×particle^-1). 以转铁蛋白修饰上述发光纳米粒子表面制备的纳米生物探针被成功应用于活的HeLa肿瘤细胞的特异性标记和双光子激发Eu³⁺发光成像.

关键词:

English

Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

1.
1 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
2 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China;
3 Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China
Received Date: 20 June 2012
Available Online: 26 September 2012
Fund Project:
国家自然科学基金(21073002, 21133001, 21227803, 51121091)

国家重点基础研究专项经费(2011CB808702)资助

Abstract:

Luminescent nanospheres (EuPHS, d_av=45 nm) containing 40% (w) of Eu(tta)₃dpbt (tta= thenoyltrifluoroacetonato;dpbt=2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine) were prepared by encapsulating Eu(tta)₃dpbt in a hybrid matrix formed in situ from poly(styrene-co-methyl methacrylate), octyltrimethoxysilane, and poly(octylsiloxane). The EuPHS are promising luminescent markers for bioanalysis because of their od dispersibility in aqueous solutions, high photostability, low cytotoxicity, and bright Eu³⁺ luminescence under excitation at long wavelengths. EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu³⁺ luminescence properties, with a visible-light excitation peak at 415 nm and an excitation window extending up to 475 nm. The quantum yield for Eu³⁺ luminescence was 0.31 (λ_ex=415 nm, T=23 ℃), and the two-photon excitation (TPE) action cross section was 5.0×10⁵ GM (1 GM=10^-50 cm⁴·s·photo^-1×particle^-1) at 830 nm. Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

双光子敏化Eu³⁺高效发光活细胞成像纳米生物探针

English

Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

双光子敏化Eu3+高效发光活细胞成像纳米生物探针

English

Nanoprobes with Enhanced Two-Photon-Sensitized Eu3+ Luminescence Properties for Live Cell Imaging

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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中国化学会秘书处

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Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

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