引用本文:
黄文, 李晓峰, 顾惕人. 核糖核酸酶A在DAB-环乙烷溶液中的活性和构象[J]. 物理化学学报,
1996, 12(04): 353-356.
doi:
10.3866/PKU.WHXB19960413
Citation: Huang Wen, Li Xiao-Feng, Gu Ti-Ren. Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution[J]. Acta Physico-Chimica Sinica, 1996, 12(04): 353-356. doi: 10.3866/PKU.WHXB19960413
Citation: Huang Wen, Li Xiao-Feng, Gu Ti-Ren. Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution[J]. Acta Physico-Chimica Sinica, 1996, 12(04): 353-356. doi: 10.3866/PKU.WHXB19960413
English
Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution
Abstract:
The activity and conformation of ribonuclerse A (RNaseA) solubilized in cyclohexane via dodecylammonium butyrate(DAB) reverse micelles were investigated. The activity of RNaseA was studied using the cytidine 2’,3’ -phosphate as the substrate, and it was found that kcat increases significantly with respect to that in water attended by an increased Km•FT-IR spectra of RNaseA in reverse micellar solution were investigated as a function of w0(= [H2O]/ [DAB]), and it was noted that the structure of RNaseA became losser in reverse micelles campared to that in aqueous solution. The relation between activity and conformation was discussed.
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