引用本文:
望天志, 吴鼎泉, 黄在银, 屈松生, 李东风, 廖展如, 万洪文. 紫色酸性磷酸酯酶模型化合物水解ATP的研究[J]. 物理化学学报,
1997, 13(07): 643-646.
doi:
10.3866/PKU.WHXB19970714
Citation: Wang Tian-Zhi, Wu Ding-Quan, Huang Zai-Yin, Qu Song-Sheng, Li Dong-Feng, Liao Zhan-Ru, Wan Hong-Wen. Studies on Model Compound of Purple Acid Phosphatases Hydrolysis of ATP[J]. Acta Physico-Chimica Sinica, 1997, 13(07): 643-646. doi: 10.3866/PKU.WHXB19970714
Citation: Wang Tian-Zhi, Wu Ding-Quan, Huang Zai-Yin, Qu Song-Sheng, Li Dong-Feng, Liao Zhan-Ru, Wan Hong-Wen. Studies on Model Compound of Purple Acid Phosphatases Hydrolysis of ATP[J]. Acta Physico-Chimica Sinica, 1997, 13(07): 643-646. doi: 10.3866/PKU.WHXB19970714
English
Studies on Model Compound of Purple Acid Phosphatases Hydrolysis of ATP
Abstract:
Studies on the thermokinetic properties of model compound of Purple acid phosphatases (Na2[Fe2(μ-O)(μ-OAC)2(IDA)2].5H2O) hydrolysis of ATP, at 37℃, pH=6.0 by using LKB-2107 Batch microcalorimentry system. The Michaelis constant Km=(2.898 ± 0.195)×10-4mol•L-1 and rate constant k2=(4.617±0.193)×10-3•s-1 have been determined. The result has been discussed.
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Key words:
-
Thermokinetic
- / Microcalorimetry
- / ATP
- / Hydrolysis
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