超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

引用本文: 付慧, 胡小键, 陈曦, 林少彬. 超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物[J]. 色谱, 2018, 36(5): 487-492. doi: 10.3724/SP.J.1123.2017.12029 shu

Citation: FU Hui, HU Xiaojian, CHEN Xi, LIN Shaobin. Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(5): 487-492. doi: 10.3724/SP.J.1123.2017.12029 shu

超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

中国疾病预防控制中心环境与健康相关产品安全所, 北京 100021
基金项目:
环保公益专项（201309045）.

摘要: 建立了高效、准确的同时测定人体尿样中多种多环芳烃代谢物的方法。取10.0 mL尿液，酶解，经固相萃取净化浓缩、0.2 μm滤膜过滤后，以Acquity UPLC^®HSS T3（100 mm×2.1 mm，1.8 μm）为分析柱，甲醇和水为流动相，采用负离子电喷雾多反应监测模式检测样品中多环芳烃代谢物的含量。12种多环芳烃代谢物在0.04~20.0 μg/L范围内线性关系良好，相关系数>0.99，方法检出限为0.01~0.41 μg/L，平均回收率为80.0%~105%，批内RSD为1.21%~9.12%，批间RSD为4.43%~19.7%。应用该方法对淮河流域某区域的100份人体尿样进行了检测，多环芳烃代谢物的检出率为98%。该方法操作简单，灵敏度高，选择性好，可同时检测尿中12种羟基多环芳烃代谢物。

关键词:

English

Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China
Received Date: 28 December 2017
Fund Project:
Commonweal and Environmental Protection Project of the Ministry of Environmental Protection of the People's Republic of China (No. 201309045).

Abstract: An efficient and accurate method was developed for the simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine. A 10.0-mL urine sample was hydrolyzed by enzyme and extracted using solid phase extraction. The metabolites were separated on an Acquity UPLC^®HSS T3 column (100 mm×2.1 mm, 1.8 μm) with a mobile phase comprising methanol and water. The analytes were detected by electrospray ionization (ESI)-MS/MS in negative mode and multiple reaction monitoring (MRM) mode. The linear ranges of the 12 metabolites were 0.04-20.0 μg/L with correlation coefficients above 0.99. The detection limits were in the range of 0.01-0.41 μg/L and the recoveries were 80.0%-105%. The intra-group relative standard deviations (RSDs) were less than 9.12% and the inter-group RSDs were less than 19.7%. This method was applied to the analysis of 100 urine samples and the results showed that the method was simple, sensitive and selective.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

English

Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

超高效液相色谱-三重四极杆质谱法同时检测人尿中12种单羟基多环芳烃代谢物

English

Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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