活体小鼠耳朵的拉曼成像方法研究

引用本文: 李雪, 高国明, 牛丽媛, 林漫漫, 覃宗定, 刘军贤, 姚辉璐. 活体小鼠耳朵的拉曼成像方法研究[J]. 分析化学, 2012, 40(10): 1494-1499. doi: 10.3724/SP.J.1096.2012.20168 shu

Citation: LI Xue, GAO Guo-Ming, NIU Li-Yuan, LIN Man-Man, QIN Zong-Ding, LIU Jun-Xian, YAO Hui-Lu. In Vivo Raman Imaging of Mice Ear[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1494-1499. doi: 10.3724/SP.J.1096.2012.20168 shu

活体小鼠耳朵的拉曼成像方法研究

通讯作者: 姚辉璐

基金项目:
本文系国家自然科学基金(Nos.60868002,31060128) (Nos.60868002,31060128)

广西自然科学基金(Nos.2012GXNSFFA060008,2011GXNSFA018143,0731013)资助 (Nos.2012GXNSFFA060008,2011GXNSFA018143,0731013)

摘要: 利用扫描技术获取活体小鼠耳朵组织不同深度的微区拉曼光谱,选取分别归属于血糖、脂类、血红蛋白、蛋白质分子结构的物质的特征谱带1125,1300,1549 和1660 cm^-1 进行峰面积计算,利用这些数据重建二维三维拉曼光谱图像。图像清晰显示了不同物质在活体组织中空间分布情况。实验表明,活体拉曼成像技术可以成为活体研究的新手段。

关键词:

光谱学
/ 拉曼
/ 成像
/ 活体

English

In Vivo Raman Imaging of Mice Ear

1.
¹ College of Physics & Technology, Guangxi Normal University, Guilin 541004, China;

Corresponding author: 姚辉璐

Received Date: 23 February 2012
Fund Project:
本文系国家自然科学基金(Nos.60868002,31060128)

广西自然科学基金(Nos.2012GXNSFFA060008,2011GXNSFA018143,0731013)资助

Abstract: Raman spectra had been used to image the spatial distribution of chemical composition by scanning the laser spot across some parts of mice ear at different depth. Selecting the bands at 1125 cm^-1(blood sugar), 1300 cm^-1(lipids), 1549 cm^-1 (hemoglobin), 1660 cm^-1 (protein) to calculate their peak areas, and using these data to generate 2D, and 3D images, we have demonstrated that Raman imaging can yield 3D spatially biochemical information from a living tissue. This technique provides a new way to study the biochemical molecule's distribution in vivo.

Key words:

Spectroscopy
/ Raman spectrum
/ Imaging
/ In vivo

1. [1]
  1 ZHANG Zheng-Xi. Biomedical Photonics New Technology and Application. Beijing: Science Press., 2008: 731 ZHANG Zheng-Xi. Biomedical Photonics New Technology and Application. Beijing: Science Press., 2008: 73
2. [2]
  张镇西. 生物医学光子学新技术及应用. 北京: 科学出版社, 2008: 73张镇西. 生物医学光子学新技术及应用. 北京: 科学出版社, 2008: 73
3. [3]
  2 Smith J, Kendall C, Sammon A, Christie-Brown J, Stone N. Techno Cancer Res Treat., 2003, 2(4): 327-3292 Smith J, Kendall C, Sammon A, Christie-Brown J, Stone N. Techno Cancer Res Treat., 2003, 2(4): 327-329
4. [4]
  3 Yao H, Tao Z, Ai M, Peng L, Wang G, He B, Li Y. Vibrational Spectroscopy, 2009, 50(2): 193-1973 Yao H, Tao Z, Ai M, Peng L, Wang G, He B, Li Y. Vibrational Spectroscopy, 2009, 50(2): 193-197
5. [5]
  4 JIANG Yang,REN Bin,TIAN Zhong-Qun. Spectroscopy and Spectral Analysis, 2008, 28(5): 1080-10904 JIANG Yang,REN Bin,TIAN Zhong-Qun. Spectroscopy and Spectral Analysis, 2008, 28(5): 1080-1090
6. [6]
  江洋,任斌,田中群. 光谱学与光谱分析, 2008, 28(5): 1080-1090江洋,任斌,田中群. 光谱学与光谱分析, 2008, 28(5): 1080-1090
7. [7]
  5 Larraona-Puy M, Ghita A, Zoladek A, Perkins W, Varma S, Leach IH, Koloydenko AA, Williams H, Notingher I. Journal of Biomedical Optics, 2009, 14(5): 054031-1-95 Larraona-Puy M, Ghita A, Zoladek A, Perkins W, Varma S, Leach IH, Koloydenko AA, Williams H, Notingher I. Journal of Biomedical Optics, 2009, 14(5): 054031-1-9
8. [8]
  6 Sasic S, Clark D, CMitchell J, Sonwden M J. Analyst, 2004, 129: 1001-10076 Sasic S, Clark D, CMitchell J, Sonwden M J. Analyst, 2004, 129: 1001-1007
9. [9]
  7 YU Ge, ZHANG Pan, TAN En-Zhong, ZHANG Cun-Zhou. Spectroscopy and Spectral Analysis, 2007, 27(2): 295-2987 YU Ge, ZHANG Pan, TAN En-Zhong, ZHANG Cun-Zhou. Spectroscopy and Spectral Analysis, 2007, 27(2): 295-298
10. [10]
  于舸, 张攀, 谭恩忠, 张存洲. 光谱学与光谱分析, 2007, 27(2): 295-298于舸, 张攀, 谭恩忠, 张存洲. 光谱学与光谱分析, 2007, 27(2): 295-298
11. [11]
  8 YuT, Ni Z H, Du C L, You Y M, Wang Y Y, Shen Z X. Journal of Physical Chemistry, 2008, 112(33): 12602-126058 YuT, Ni Z H, Du C L, You Y M, Wang Y Y, Shen Z X. Journal of Physical Chemistry, 2008, 112(33): 12602-12605
12. [12]
  9 HUANG Qiao-Song,YU Zhao-Xian,LI Jing. Journal of Physical Chemistry, 2008, 28(12): 2880-28849 HUANG Qiao-Song,YU Zhao-Xian,LI Jing. Journal of Physical Chemistry, 2008, 28(12): 2880-2884
13. [13]
  黄乔松, 于肇贤, 李静. 光谱学与光谱分析, 2008, 28(12): 2880-2884黄乔松, 于肇贤, 李静. 光谱学与光谱分析, 2008, 28(12): 2880-2884
14. [14]
  10 Keren S, Zavaleta C, Cheng Z, de la zerda A, Gheysens O, Gambhir S S. PNAS, 2008, 105(15): 5844-584910 Keren S, Zavaleta C, Cheng Z, de la zerda A, Gheysens O, Gambhir S S. PNAS, 2008, 105(15): 5844-5849
15. [15]
  11 MENG Ling-Jing, JI Xiao-Lu, LI Zi-Da, LIU Jun-Xian, LI Lei, YAO Hui-Lu. Laser & Optoelectronics Progres., 2011, 2: 56-6011 MENG Ling-Jing, JI Xiao-Lu, LI Zi-Da, LIU Jun-Xian, LI Lei, YAO Hui-Lu. Laser & Optoelectronics Progres., 2011, 2: 56-60
16. [16]
  孟令晶, 纪晓露, 李自达, 刘军贤, 李蕾, 姚辉璐. 激光与光电子学进展, 2011, 2: 56-60孟令晶, 纪晓露, 李自达, 刘军贤, 李蕾, 姚辉璐. 激光与光电子学进展, 2011, 2: 56-60
17. [17]
  12 Stone N, Kendall C, Smith J, Crow P, Barr H. Faraday Discuss, 2004, 126: 141-15712 Stone N, Kendall C, Smith J, Crow P, Barr H. Faraday Discuss, 2004, 126: 141-157
18. [18]
  13 Wood B R, Tait B, McNaughton D. BBA, 2001, 1539: 58-7013 Wood B R, Tait B, McNaughton D. BBA, 2001, 1539: 58-70
19. [19]
  14 XieC G, Li Y Q, Tang W, Ronald J. Journal of Applied Physics, 2003, 94(9): 613814 XieC G, Li Y Q, Tang W, Ronald J. Journal of Applied Physics, 2003, 94(9): 6138

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

活体小鼠耳朵的拉曼成像方法研究

通讯作者: 姚辉璐

English

In Vivo Raman Imaging of Mice Ear

Corresponding author: 姚辉璐

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

活体小鼠耳朵的拉曼成像方法研究

通讯作者: 姚辉璐

English

In Vivo Raman Imaging of Mice Ear

Corresponding author: 姚辉璐

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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