酸性红73多克隆抗体的制备及其应用

引用本文: 常向彩, 杨晓农, 宋定州, 刘倩, 于学辉, 程江, 黄伟. 酸性红73多克隆抗体的制备及其应用[J]. 分析化学, 2012, 40(10): 1593-1597. doi: 10.3724/SP.J.1096.2012.20115 shu

Citation: CHANG Xiang-Cai, YANG Xiao-Nong, SONG Ding-Zhou, LIU Qian, YU Xue-Hui, CHENG Jiang, HUANG Wei. Preparation and Application of Acid Red 73 Polyclonal Antibody[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1593-1597. doi: 10.3724/SP.J.1096.2012.20115 shu

酸性红73多克隆抗体的制备及其应用

常向彩 ^1, ,
杨晓农 ^{1,
,} ,
宋定州 ^1, ,
刘倩 ^1, ,
于学辉 ^1, ,
程江 ^2, ,
黄伟 ^1,

通讯作者: 杨晓农

基金项目:
本文系西南民族大学研究生创新型科研重点项目(No.CX2011SZ16) (No.CX2011SZ16)

西南民族大学研究生学位点建设项目(No.2011XWD-S0906)资助 (No.2011XWD-S0906)

摘要: 在酸性红73分子的羟基上引入一个带有羧基的"间隔臂",采用N-羟基琥珀亚胺活性酯法将酸性红73分别与牛血清白蛋白(BSA)、卵清蛋白(OA)偶联,合成免疫原和包被原,经免疫新西兰白兔获得多克隆抗体,所得抗体最大效价可达2.56×10⁵,建立了酸性红73的间接竞争ELISA检测方法。本方法的半数抑制浓度(IC₅₀)为181.2 μg/L,检出限(LOD)为7.9 μg/L。交叉反应实验表明,除苏丹红3号(1.13%)外,抗AR73抗体与其它竞争物均无交叉反应。在虾仁中的空白添加回收率为63.5%-90.7%,RSD＜6.8%。说明本方法可用于虾仁中酸性红73的残留检测。

关键词:

English

Preparation and Application of Acid Red 73 Polyclonal Antibody

1.
¹ College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;

Corresponding author: 杨晓农

Received Date: 13 February 2012
Fund Project:
本文系西南民族大学研究生创新型科研重点项目(No.CX2011SZ16)

西南民族大学研究生学位点建设项目(No.2011XWD-S0906)资助

Abstract: Connecting the hydroxyl of acid red 73 (AR73) molecule with a "spacer arm" containing a carboxyl as hapten, and the hapten was conjugated to bovine serum albumin (BSA) or ovalbumin (OA) by using N-hydroxysuccinimide active ester method as immunogen and coating antigen respectively, the polyclonal antibody titer was 2.56×10⁵ through immunization of New zealand white rabbits, then an indirect ELISA procedure for the determination of acid red 73 was established. The half of inhibition concentration (IC₅₀) was 181.2 μg/L, the limit of detection for acid red 73 was 7.9 μg/L, and the cross-reactivity study showed that the polyclonal antiserum was highly specific to AR73, and no cross-reactivity was detected between the obtained polyclonal antiserum and the other competitors except to Sudan red Ⅲ (1.13%). The recoveries from the standards fortified blank samples were in the range of 63.5%-90.7% with RSD lower than 6.8%. The developed method can be used to determine the acid red 73 residue in shrimps, and can help to prepare the monoclonal antibody and develop the rapid test kits for acid red 73.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

酸性红73多克隆抗体的制备及其应用

通讯作者: 杨晓农

English

Preparation and Application of Acid Red 73 Polyclonal Antibody

Corresponding author: 杨晓农

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

酸性红73多克隆抗体的制备及其应用

通讯作者: 杨晓农

English

Preparation and Application of Acid Red 73 Polyclonal Antibody

Corresponding author: 杨晓农

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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