引用本文:
谭克俊, 马璐, 覃明丽. 基于与Ce3+相互作用共振光散射法测定三磷酸腺苷二钠[J]. 应用化学,
2010, 27(6): 737-741.
doi:
10.3724/SP.J.1095.2010.90548
Citation: TAN Ke-Jun, MA Lu, QIN Ming-Li. Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+[J]. Chinese Journal of Applied Chemistry, 2010, 27(6): 737-741. doi: 10.3724/SP.J.1095.2010.90548
Citation: TAN Ke-Jun, MA Lu, QIN Ming-Li. Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+[J]. Chinese Journal of Applied Chemistry, 2010, 27(6): 737-741. doi: 10.3724/SP.J.1095.2010.90548
基于与Ce3+相互作用共振光散射法测定三磷酸腺苷二钠
English
Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+
Abstract:
In Tris-HCl buffer solution,a resonance light scattering(RLS) detection method for adenosine disodium triphosphate(ATP) was developed based on RLS signal enhancement due to interactions of ATP with cerium ions in an alkalescent aqueous medium.The enhanced RLS intensity was proportional to ATP content in a range of 2.0~24 μmol/L with a limit of determination(LOD,S/N=3σ) of 51.25 nmol/L.The effects of coexisting substances were investigated.The method has been successfully applied to determining the content of ATP in injection samples with a RSD of 2.29%.
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Key words:
- adenosine disodium triphosphate
- / cerium ions
- / resonance light scattering
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