Abstract: 2,6-Di(2'-imidazoline-2'-yl)pyridine,even though it is not fully aromatic,has the same distribution of heteroatoms as 2,6-di(2'-benzimidazol-2'-yl)pyridine and has been synthesized from pyridine 2,6-dicarboxylic acid and ethylenediamine under microwave irradiation and without catalyst. The composition and structure of the product were identified and characterized by IR,mass spectrometry,¹H NMR,¹³C NMR and melting point determination. The ratio of products and by-product was found to be different under different experimental conditions(solvents,proportion of reacting mass,power of microwave irradiation). Among them,solvents have very obvious effects on the ratio of product and by-product. The experimental results indicate that the best solvent is ethylene glycol. The optimal reaction condition are follows:molar ratio of raw materials 1:2.7,microwave power 450 W,irradiation time 65 min,irradiation temperature 130℃ and the overall yield 71.2%.The binding of this compound with calf thymus DNA was investigated by UV and viscosity measurements.The binding constant K_b is meaused to be(9.60±0.05)×10⁴ L/mol,which is lower than those observed for typical classical intercalators.The results of viscosity measurement reveal that the presence of 2,6-di(2'-imidazoline-2'-yl)pyridine has no obvious effect on the relative viscosity of DNA.It may bind to DNA by hydrogen bonding and groove-face binding.