硅表面分子刷图案化及生长DNA纳米管

引用本文: 鄢琴, 肖守军. 硅表面分子刷图案化及生长DNA纳米管[J]. 无机化学学报, 2014, 30(4): 741-748. doi: 10.11862/CJIC.2014.134 shu

Citation: YAN Qin, XIAO Shou-Jun. In-situ Growth of DNA Nanotubes from Functionalized Patterns of Poly(PEGMA) Brushes on Silicon Surface[J]. Chinese Journal of Inorganic Chemistry, 2014, 30(4): 741-748. doi: 10.11862/CJIC.2014.134 shu

硅表面分子刷图案化及生长DNA纳米管

鄢琴 ,
肖守军 ^,

通讯作者: 肖守军 sjxiao@nju.edu.cn

基金项目:
国家自然科学基金(No.91027019)资助项目。 (No.91027019)

摘要: 结合“自上而下”和“自下而上”技术构建微纳米器件是目前纳米科学和技术领域追逐的目标之一。本文首先采用硅氢化反应在硅表面共价偶联引发聚合的活性基团,接着实施表面原子转移自由基聚合(ATRP)反应形成高分子刷poly(PEGMA),采用“自上而下”的光刻技术在硅表面制备功能化的图案,最后利用“自下而上”的DNA自组装技术在图案部分原位生长DNA纳米管。上述组装过程通过多次透射反射红外光谱、凝胶电泳、透射电镜和扫描电镜进行了检测,证实了硅芯片表面定位生长DNA纳米管的可行性。

关键词:

自组装;光刻;聚合物刷;DNA纳米管

English

In-situ Growth of DNA Nanotubes from Functionalized Patterns of Poly(PEGMA) Brushes on Silicon Surface

YAN Qin ,
XIAO Shou-Jun ^,

1.
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

Corresponding author: 肖守军 sjxiao@nju.edu.cn

Received Date: 23 July 2013
Fund Project:
国家自然科学基金(No.91027019)资助项目。

Abstract: Interfacing "top-down" with "bottom-up" methods to fabricate micro-and nano-devices are one target in nano-science and nano-technology presently. Herein we demonstrate the combination of photolithography and self-assembly to grow DNA nanotubes on functionalized patterns of poly(poly(ethylene glycol)monomethacrylate) brushes grafted from a silicon chip surface (Si-g-Poly(PEGMA)). On the silicon surface, first, the bromoisobutyryl group as the polymerization initiator was introduced through hydrosilylation, then poly(PEGMA) brushes were grown in-situ by Surface Initiated Atom Transfer Radical Polymerization (SI-ATRP), NHS(succinimidyl)-ester was generated on photolithographied poly(PEGMA) patterns and finally six-helix DNA tubes were immobilized and grown in-situ on these patterns. Multiple transmission-reflection infrared spectroscopy (MTR-IR), gel electrophoresis, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to monitor the whole process, which confirmed the feasibility of DNA self-assembly on the functionalized patterns of a silicon chip.

Key words:

DNA nanotube;self-assembly;photolithography;polymer brushes

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

硅表面分子刷图案化及生长DNA纳米管

通讯作者: 肖守军 sjxiao@nju.edu.cn

English

In-situ Growth of DNA Nanotubes from Functionalized Patterns of Poly(PEGMA) Brushes on Silicon Surface

Corresponding author: 肖守军 sjxiao@nju.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

硅表面分子刷图案化及生长DNA纳米管

通讯作者: 肖守军 sjxiao@nju.edu.cn

English

In-situ Growth of DNA Nanotubes from Functionalized Patterns of Poly(PEGMA) Brushes on Silicon Surface

Corresponding author: 肖守军 sjxiao@nju.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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