引用本文:
Ken Zhu, Ke An LI, Shen Yang TONG. A Study on the Reaction Mechanism of Bromocresol Green with Bovine Serum Albumin[J]. Chinese Chemical Letters,
1996, 7(11): 1029-1032.
Citation: Ken Zhu, Ke An LI, Shen Yang TONG. A Study on the Reaction Mechanism of Bromocresol Green with Bovine Serum Albumin[J]. Chinese Chemical Letters, 1996, 7(11): 1029-1032.
Citation: Ken Zhu, Ke An LI, Shen Yang TONG. A Study on the Reaction Mechanism of Bromocresol Green with Bovine Serum Albumin[J]. Chinese Chemical Letters, 1996, 7(11): 1029-1032.
A Study on the Reaction Mechanism of Bromocresol Green with Bovine Serum Albumin
摘要:
The protein assay with bromocresol green(BCG)method is commonly used because of its convenience,but it is not well understood on a molecular level.In this paper,the study on the reaction between BCG and bovine serum albumin(BSA)is reported.The binding numbers and the binding constants are given at different pH by using three methods with relevant comparison.It was proved that there were three sorts of binding sites of BSA to react with BCG and BCG transformed into its basic form in the presence of BSA.
English
A Study on the Reaction Mechanism of Bromocresol Green with Bovine Serum Albumin
Abstract:
The protein assay with bromocresol green(BCG)method is commonly used because of its convenience,but it is not well understood on a molecular level.In this paper,the study on the reaction between BCG and bovine serum albumin(BSA)is reported.The binding numbers and the binding constants are given at different pH by using three methods with relevant comparison.It was proved that there were three sorts of binding sites of BSA to react with BCG and BCG transformed into its basic form in the presence of BSA.
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