Abstract: A novel extracting agent for solid phase extraction was prepared by immobilizing dye ligand onto the surface of nonporous Urea-Formaldehyde resin-Zirconia composite microspheres via nucleophilic reaction,where the amido groups on the surface of the microspheres reacted with the active chloride in the dye.Bovine serum albumin (BSA) was chosen as the testing sample,and the kinetics and thermodynamics of BSA adsorption on the newly synthesized extracting agent were investigated under different conditions.The result indicates that the extraction capacity of the extracting agent reached 34.5 mg/g,and the extraction can be described by Langmuir adsorption isotherm.The extraction amount reached a maximum at pH=4.6,and when the experiment was carried out for 2 hours,the extraction amount reached 80% of the maximum.The extracted protein was easily eluted by 1 mol/L KSCN,and the elution efficiency was 93.5%,and also the elution was reproducible.This extracting agent could be used to separate interferential proteins and enrich target protein before HPLC analysis.