引用本文:
李春英, 赵春建, 祖元刚. 高效液相色谱法同时测定烟叶中茄尼醇和辅酶Q10的含量[J]. 应用化学,
2006, 23(5): 524-527.
Citation: LI Chun-Ying, ZHAO Chun-Jian, ZU Yuan-Gang. Simultaneous Determination of Solanesol and Coenzyme Q10 in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC[J]. Chinese Journal of Applied Chemistry, 2006, 23(5): 524-527.
Citation: LI Chun-Ying, ZHAO Chun-Jian, ZU Yuan-Gang. Simultaneous Determination of Solanesol and Coenzyme Q10 in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC[J]. Chinese Journal of Applied Chemistry, 2006, 23(5): 524-527.
高效液相色谱法同时测定烟叶中茄尼醇和辅酶Q10的含量
摘要:
建立了一种同时测定烟叶中茄尼醇和辅酶Q10的反相高效液相色谱法。以匀浆萃取法作为样品的制备方法。色谱条件为:HIQ SIL C18V色谱柱(4.6 mm×250 mm,5 μm),柱温25℃;流动相为V(乙腈):V(异丙醇)=8:7,流速1.5 mL/min;使用二级管阵列检测器,茄尼醇检测波长为215 nm,辅酶Q10检测波长为275 nm;进样量20 μL。在该色谱条件下,茄尼醇和辅酶Q10的回收率分别为(100.05±1.25)%和(99.97±1.36)%。方法简单、快速、高效,适合于烟叶中茄尼醇和辅酶Q10的测定。
English
Simultaneous Determination of Solanesol and Coenzyme Q10 in the Extract of Tobacco(Nicotiana Tabacum L.) Leaves by HPLC
Abstract:
The simultaneous determination of solanesol and coenzyme Q10 in the extract of tobacco(Nicotiana Tabacum L.) leaves by using reversed phase high performance liquid chromatography(RP-HPLC) method was established.The sample preparation process involved homogeneous extraction.The chromatogram was obtained on an HIQ SIL C18V column(4.6 mm×250 mm,5 μm) with V(acetonitrile):V(isopropanol)=8:7 as the mobile phase at a flow rate of 1.5 mL/min and 25℃.Diode array detection(DAD) was used and UV detection wave wavelength was set at 215 nm for solanesol and 275 nm for coenzyme Q10. The volume of injection sample was 20 μL.Under the chromatographic conditions,the recoveries of solanesol and coenzyme Q10 were(100.05±1.25)% and (99.97±1.36)% respectively.The method is simple,rapid,efficient and it is suitable to the determination of solanesol and coenzyme Q10 in the extract of tobacco leaves.
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Key words:
- RP-HPLC
- / DAD
- / tobacco leaves
- / solanesol
- / coenzyme Q10
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