引用本文:
王鹏, 王玉红, 冯楠, 刘东晖, 江树人, 周志强. 淀粉衍生物固定相对马拉硫磷和氟虫腈对映体的手性拆分[J]. 应用化学,
2006, 23(10): 1170-1172.
Citation: WANG Peng, WANG Yu-Hong, FENG Nan, LIU Dong-Hui, JIANG Shu-Ren, ZHOU Zhi-Qiang. Chiral Resolution of Malathion and Fipronil on the Amylase Derivative Chiral Stationary Phase[J]. Chinese Journal of Applied Chemistry, 2006, 23(10): 1170-1172.
Citation: WANG Peng, WANG Yu-Hong, FENG Nan, LIU Dong-Hui, JIANG Shu-Ren, ZHOU Zhi-Qiang. Chiral Resolution of Malathion and Fipronil on the Amylase Derivative Chiral Stationary Phase[J]. Chinese Journal of Applied Chemistry, 2006, 23(10): 1170-1172.
淀粉衍生物固定相对马拉硫磷和氟虫腈对映体的手性拆分
摘要:
对马拉硫磷和氟虫腈2种手性杀虫剂进行了对映体的高效液相色谱拆分研究,固定相为直链淀粉-三((S)-1-苯基乙基氨基甲酸酯),流动相为正己烷,流速1.0 mL/min,检测波长230 nm。使用5种醇作为极性的改性剂,在正己烷/异丙醇流动相条件下,马拉硫磷最大分离度为0.85,氟虫腈2对映体可以实现基线分离,最大分离度达1.98。利用圆二色检测器测定了对映体的出峰顺序,在230 nm波长下,马拉硫磷的出峰顺序为+/-,而氟虫腈的出峰顺序则为-/+,并在220~420 nm之间测定了对映体的圆二色谱。
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关键词:
- 马拉硫磷
- / 氟虫腈
- / 手性拆分
- / 直链淀粉-三((S)-苯基乙基氨基甲酸酯)
English
Chiral Resolution of Malathion and Fipronil on the Amylase Derivative Chiral Stationary Phase
Abstract:
The chiral resolution of malathion and fipronil enantiomers was performed by high-performance liquid chromatography with amylose tri-(S)-1-phenylethylcarbamate as chiral stationary phase.The mobile phase was n-hexane,with ethanol,1-propanol,2-propanol,1-butanol or 2-butanol used as polar modifiers.The flow rate was 1.0 mL/min and detecting wavelength was 230 nm.The best resolution factor for malathion was 0.85 with n-hexane/isopropanol as the mobile phase.The two enantiomers of fipronil could achieve baseline separation with the best resolution factor of 1.98.The optical rotations were determined by a circular dichroism detector.The elution order for malathion was +/- and that of fipronil was -/+ at 230 nm.The circular dichroism spectra for the enantiomers were also studied.
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Key words:
- malathion
- / fipronil
- / chiral resolution
- / amylose tri-(S)-1-phenylethylcarbamate
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