引用本文:
康天放, 刘润, 鲁理平, 程水源. 再生丝素固定乙酰胆碱酯酶生物传感器[J]. 应用化学,
2006, 23(10): 1099-1103.
Citation: KANG Tian-Fang, LIU Run, LU Li-Ping, CHENG Shui-Yuan. Biosensors Based on Acetylcholinesterase Immobilized on Regenerated Silk Fibroin[J]. Chinese Journal of Applied Chemistry, 2006, 23(10): 1099-1103.
Citation: KANG Tian-Fang, LIU Run, LU Li-Ping, CHENG Shui-Yuan. Biosensors Based on Acetylcholinesterase Immobilized on Regenerated Silk Fibroin[J]. Chinese Journal of Applied Chemistry, 2006, 23(10): 1099-1103.
再生丝素固定乙酰胆碱酯酶生物传感器
English
Biosensors Based on Acetylcholinesterase Immobilized on Regenerated Silk Fibroin
Abstract:
An amperometric biosensor for the determination of organophosphorus and carbamate pesticides is described.Acetylcholinesterase(AChE) immobilized on regenerated silk fibroin powder was thoroughly mixed with graphite powder,non-conducting epoxy resin,and electronic mediator 7,7,8,8-tetracyanoquinodimethane(TCNQ) to prepare the biosensor.The preparation conditions of the biosensor were optimized.The proposed method exhibited a good reproducibility and recovery ratio.The inhibition versus the logarithm of concentration was linear to omethoate and carbofuran over the concentration ranges of 5.0×10-5 g/L to 5.0×10-3 mg/L and 5.0×10-5 g/L to 1.0 mg/L with the detection limits of 1.9×10-5 mg/L and 4.7×10-5 mg/L,respectively.
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Key words:
- biosensor
- / acetylcholinesterase
- / regenerated silk fibroin
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