引用本文:
邵承斌, 郑旭煦, 李新平. 黑麦草叶片超氧化物歧化酶[J]. 应用化学,
2003, 20(5): 474-478.
Citation: SHAO Cheng-Bin, ZHENG Xu-Xu, LI Xin-Ping. Superoxide Dismutase from Ryegrass Leaves (Lolium perenneperennial)[J]. Chinese Journal of Applied Chemistry, 2003, 20(5): 474-478.
Citation: SHAO Cheng-Bin, ZHENG Xu-Xu, LI Xin-Ping. Superoxide Dismutase from Ryegrass Leaves (Lolium perenneperennial)[J]. Chinese Journal of Applied Chemistry, 2003, 20(5): 474-478.
黑麦草叶片超氧化物歧化酶
English
Superoxide Dismutase from Ryegrass Leaves (Lolium perenneperennial)
Abstract:
Superoxide dismutase (SOD) separated and extracted from ryegrass leaves (Lolium perenneperennial) was purified by fraction precipitation by ammonium sulfate and column chromatography.The purified enzyme is found to be a copper/zinc superoxide dismutase as assayed by its sensitivity to KCN and H2O2.Electrophoretic analysis indicated that the enzyme contains two homogenous subunits with molecular weight of 15900 with N-terminal being alanine and maximum ultraviolet absorption at 254 nm.The SOD from ryegrass leaves is thermal stable with activity loss of 27% at 65℃ for an hour.Quantitative analysis of amino acids revealed that this protein was composed of 212 amino acids and lack of tryptophan.
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Key words:
- superoxide dismutase
- / ryegrass leaves
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